Literature DB >> 2987645

The Klebsiella aerogenes glutamate dehydrogenase (gdhA) gene: cloning, high-level expression and hybrid enzyme formation in Escherichia coli.

A Mountain, M J McPherson, A J Baron, J C Wootton.   

Abstract

The NADP-dependent glutamate dehydrogenase gene of Klebsiella aerogenes was cloned in E. coli in the expression plasmid pRK9. The cloned gene shows a high level of expression in E. coli in the hybrid plasmid pKG3 and such expression is independent of the vector promoter, as shown by experiments in which the promoter was deleted. Active hybrid GDH hexamers were shown in cell-free extracts of an E. coli strain carrying cloned gdhA genes of both E. coli and K. aerogenes. The nucleotide sequence of the N-terminal coding region of the K. aerogenes gdhA gene was determined and found to be strongly homologous with that of E. coli.

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Year:  1985        PMID： 2987645     DOI： 10.1007/bf00327523

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  30 in total

1.  Regulation of enzyme formation in Klebsiella aerogenes by episomal glutamine synthetase of Escherichia coli.

Authors:  S L Streicher; A B Deleo; B Magasanik
Journal:  J Bacteriol       Date:  1976-07       Impact factor: 3.490

2.  Affinity chromatography of the Neurospora NADP-specific glutamate dehydrogenase, its mutational variants and hybrid hexamers.

Authors:  D H Watson; J C Wootton
Journal:  Biochem J       Date:  1977-10-01       Impact factor: 3.857

3.  Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.

Authors:  N E Murray; S A Bruce; K Murray
Journal:  J Mol Biol       Date:  1979-08-15       Impact factor: 5.469

4.  ColE1 hybrid plasmids containing Escherichia coli genes involved in the biosynthesis of glutamate and glutamine.

Authors:  A A Covarrubias; R Sánchez-Pescador; A Osorio; F Bolivar; F Bastarrachea
Journal:  Plasmid       Date:  1980-03       Impact factor: 3.466

5.  Rapid and efficient cosmid cloning.

Authors:  D Ish-Horowicz; J F Burke
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

6.  Localisation of a strongly conserved section of coding sequence in glutamate dehydrogenase genes.

Authors:  I W Mattaj; M J McPherson; J C Wootton
Journal:  FEBS Lett       Date:  1982-10-04       Impact factor: 4.124

7.  Regulation of the synthesis of enzymes responsible for glutamate formation in Klebsiella aerogenes.

Authors:  J E Brenchley; M J Prival; B Magasanik
Journal:  J Biol Chem       Date:  1973-09-10       Impact factor: 5.157

8.  Cloning of the am (glutamate dehydrogenase) gene of Neurospora crassa through the use of a synthetic DNA probe.

Authors:  J H Kinnaird; M A Keighren; J A Kinsey; M Eaton; J R Fincham
Journal:  Gene       Date:  1982-12       Impact factor: 3.688

9.  Re-assessment of ammonium-ion affinities of NADP-specific glutamate dehydrogenases. Activation of the Neurospora crassa enzyme by ammonium and rubidium ions.

Authors:  J C Wootton
Journal:  Biochem J       Date:  1983-02-01       Impact factor: 3.857

10.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469
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  9 in total

1.  Growth inhibition caused by overexpression of the structural gene for glutamate dehydrogenase (gdhA) from Klebsiella aerogenes.

Authors:  B K Janes; P J Pomposiello; A Perez-Matos; D J Najarian; T J Goss; R A Bender
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

2.  Nucleotide sequence and regulation of expression of the Aspergillus nidulans gdhA gene encoding NADP dependent glutamate dehydrogenase.

Authors:  A R Hawkins; S J Gurr; P Montague; J R Kinghorn
Journal:  Mol Gen Genet       Date:  1989-07

3.  The gdhA1 point mutation in Escherichia coli K12 CLR207 alters a key lysine residue of glutamate dehydrogenase.

Authors:  K M Jones; M J McPherson; A J Baron; I W Mattaj; C L Riordan; J C Wootton
Journal:  Mol Gen Genet       Date:  1993-08

4.  A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana.

Authors:  J M Cock; K D Kim; P W Miller; R G Hutson; R R Schmidt
Journal:  Plant Mol Biol       Date:  1991-11       Impact factor: 4.076

5.  The gene coding for glutamate dehydrogenase in Drosophila melanogaster.

Authors:  D Papadopoulou; C Louis
Journal:  Biochem Genet       Date:  1990-08       Impact factor: 1.890

6.  Isolation and identification of the Aspergillus nidulans gdhA gene encoding NADP-linked glutamate dehydrogenase.

Authors:  S J Gurr; A R Hawkins; C Drainas; J R Kinghorn
Journal:  Curr Genet       Date:  1986       Impact factor: 3.886

7.  The NADP-glutamate dehydrogenase of the cyanobacterium Synechocystis 6803: cloning, transcriptional analysis and disruption of the gdhA gene.

Authors:  S Chávez; J C Reyes; F Chauvat; F J Florencio; P Candau
Journal:  Plant Mol Biol       Date:  1995-04       Impact factor: 4.076

8.  Expression of the Escherichia coli glutamate dehydrogenase gene in the cyanobacterium Synechococcus PCC6301 causes ammonium tolerance.

Authors:  D A Lightfoot; A J Baron; J C Wootton
Journal:  Plant Mol Biol       Date:  1988-05       Impact factor: 4.076

9.  Collagen-like sequences stabilize homotrimers of a bacterial hydrolase.

Authors:  B M Charalambous; J N Keen; M J McPherson
Journal:  EMBO J       Date:  1988-09       Impact factor: 11.598
  9 in total

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