Savvina Chortarea1,2, Kleanthis Fytianos1,3,4, Laura Rodriguez-Lorenzo1, Alke Petri-Fink1,5, Barbara Rothen-Rutishauser1. 1. BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland. 2. Laboratory for Particles - Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland. 3. Department of Pulmonary Medicine, University of Bern, Bern, Switzerland. 4. Department of Clinical Research, University of Bern, Bern, Switzerland. 5. Department of Chemistry, University of Fribourg, Fribourg, Switzerland.
Abstract
AIM: The distribution and impact of aerosol-delivered gold nanoparticles (AuNPs) functionalized with a mixture of aminated-polyvinyl alcohol and amino-PEG ([polyvinyl alcohol/PEG]-NH2) upon repeated administration onto a 3D lung model were explored. MATERIALS & METHODS: AuNPs were aerosolized and uptake and epithelial translocation was assessed by inductively coupled plasma optical-emission spectroscopy, flow cytometry and electron microscopy. In addition, cytotoxicity, apoptosis and proinflammation were evaluated. RESULTS: Repeated AuNP aerosolization resulted in NP accumulation in macrophages and epithelial cells. Dendritic cells demonstrated substantial NP internalization after single administration which was reduced in later time points. No cytotoxicity or proinflammation was observed but after 96 h significant apoptosis was induced by the polymer coating. CONCLUSION: These results indicate the importance of repeated exposures in addressing potential effects of NPs.
AIM: The distribution and impact of aerosol-delivered gold nanoparticles (AuNPs) functionalized with a mixture of aminated-polyvinyl alcohol and amino-PEG ([polyvinyl alcohol/PEG]-NH2) upon repeated administration onto a 3D lung model were explored. MATERIALS & METHODS: AuNPs were aerosolized and uptake and epithelial translocation was assessed by inductively coupled plasma optical-emission spectroscopy, flow cytometry and electron microscopy. In addition, cytotoxicity, apoptosis and proinflammation were evaluated. RESULTS: Repeated AuNP aerosolization resulted in NP accumulation in macrophages and epithelial cells. Dendritic cells demonstrated substantial NP internalization after single administration which was reduced in later time points. No cytotoxicity or proinflammation was observed but after 96 h significant apoptosis was induced by the polymer coating. CONCLUSION: These results indicate the importance of repeated exposures in addressing potential effects of NPs.
Entities:
Keywords:
air–liquid interface; biodistribution; biomedical gold nanoparticles; hazard assessment; in vitro 3D lung system; short-term repeated aerosol exposure