Hanyan Liu1, Jianqiao Liu1, Shiping Chen1, Xiangjin Kang1, Hongzi Du1, Lei Li2. 1. Center for Reproductive Medicine, Third Affiliated Hospital of Guangzhou Medical University; Key Laboratory for Reproductive Medicine of Guangdong; Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes; and Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, People's Republic of China. 2. Center for Reproductive Medicine, Third Affiliated Hospital of Guangzhou Medical University; Key Laboratory for Reproductive Medicine of Guangdong; Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes; and Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou, People's Republic of China. Electronic address: lileigo@foxmail.com.
Abstract
OBJECTIVE: To identify the incidence and risk factors associated with IVF-conceived monozygotic twinning (MZT). DESIGN: Retrospective study. SETTING: Academic hospital. PATIENT(S): A total of 3,463 women with clinical pregnancies between January 2014 and February 2015 were analyzed. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The measures were the incidence of MZT based on the number of embryos that were replaced, type of insemination method (conventional IVF or intracytoplasmic sperm injection [ICSI]), with or without the use of assisted hatching (AH), and day of embryo transferred in fresh and frozen cycles. RESULT(S): Ninety-three women (2.69%) with MZT were observed. No statistically significant differences were observed in the cycle parameters of fresh or frozen cycles between MZT and other non-MZT pregnancies. Specific IVF procedures or techniques, such as the number of embryo replaced, zona pellucida manipulation (ICSI and AH), and freeze-thaw procedure, did not significantly increase the rate of MZT, except for the day of embryo transferred. Compared with day 3 transferred, day 4 and 5/6 transferred showed an increased probability of MZT (odds ratio [OR], 2.73; 95% confidence interval [CI], 1.16-6.42 for day 4 transferred and OR, 3.68; 95% CI, 2.29-5.93 for day 5/6 transferred). CONCLUSION(S): Extended culture (advanced embryo stage) in fresh and frozen cycles appeared to be associated with increased rates of MZT. The effect of the number of embryos transferred, ICSI and AH, and freeze-thaw procedures on the risk for MZT was not demonstrated.
OBJECTIVE: To identify the incidence and risk factors associated with IVF-conceived monozygotic twinning (MZT). DESIGN: Retrospective study. SETTING: Academic hospital. PATIENT(S): A total of 3,463 women with clinical pregnancies between January 2014 and February 2015 were analyzed. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The measures were the incidence of MZT based on the number of embryos that were replaced, type of insemination method (conventional IVF or intracytoplasmic sperm injection [ICSI]), with or without the use of assisted hatching (AH), and day of embryo transferred in fresh and frozen cycles. RESULT(S): Ninety-three women (2.69%) with MZT were observed. No statistically significant differences were observed in the cycle parameters of fresh or frozen cycles between MZT and other non-MZT pregnancies. Specific IVF procedures or techniques, such as the number of embryo replaced, zona pellucida manipulation (ICSI and AH), and freeze-thaw procedure, did not significantly increase the rate of MZT, except for the day of embryo transferred. Compared with day 3 transferred, day 4 and 5/6 transferred showed an increased probability of MZT (odds ratio [OR], 2.73; 95% confidence interval [CI], 1.16-6.42 for day 4 transferred and OR, 3.68; 95% CI, 2.29-5.93 for day 5/6 transferred). CONCLUSION(S): Extended culture (advanced embryo stage) in fresh and frozen cycles appeared to be associated with increased rates of MZT. The effect of the number of embryos transferred, ICSI and AH, and freeze-thaw procedures on the risk for MZT was not demonstrated.