Süleyman Oyan1, Arzu Tatlıpınar2, Beste M Atasoy3, Pembegül Güneş4, Dilek Özbeyli5, Serhan Keskin1, Ayse Dağlı Değerli6. 1. Ear Nose and Throat Clinic, Health Sciences University Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Turkey. 2. Ear Nose and Throat Clinic, Health Sciences University Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Turkey. arzutatli@yahoo.com. 3. Department of Radiation Oncology, Marmara University School of Medicine, Istanbul, Turkey. 4. Pathology Clinic, Health Sciences University Haydarpaşa Numune Training and Research Hospital, Istanbul, Turkey. 5. Experimental Animal Laboratory, Marmara University School of Medicine, Istanbul, Turkey. 6. Clinic of Radiation Oncology, Ministry of Health-Marmara University Pendik Training and Research Hospital, Istanbul, Turkey.
Abstract
OBJECTIVE: The aim of this study was to evaluate the early histopathological changes of gastroesophageal reflux and irradiation on laryngeal mucosa in rats. STUDY DESIGN: Animal study. SETTING: Experimental animal laboratory, tertiary referral center. SUBJECT AND METHOD: Twenty-four adult female Wistar Albino rats were grouped as: control (n = 6), reflux and irradiation (n = 10), and irradiation (n = 8). Rats were operated to create a reflux model 30 days before irradiation. Ionizing radiation was administered in a single fraction of a 20 Gy to the larynx. Laryngeal tissue samples were taken at the 4th day of irradiation and all specimens underwent histopathological examination. RESULTS: Edema and vascular dilation in lamina propria were higher in the reflux and irradiation, and irradiation groups than control group. Inflammation was higher in the reflux and irradiation group than the control group. Inflammation in squamous epithelium was higher in the reflux and irradiation and irradiation groups compared to the control group. Inflammation in the squamous epithelium of the irradiation group was higher than the reflux and irradiation group. In the respiratory tract epithelium, inflammation was higher in the reflux and irradiation group; additionally, a significant loss of cilia was present in the reflux and irradiation and irradiation groups while pseudostratification was higher in the reflux and irradiation group. CONCLUSION: Ionizing radiation-induced inflammation may increase on previously inflammated area due to gastroesophageal reflux. Therefore, it may be helpful to investigate and treat the reflux in laryngeal cancer patients that will receive ionizing radiation.
OBJECTIVE: The aim of this study was to evaluate the early histopathological changes of gastroesophageal reflux and irradiation on laryngeal mucosa in rats. STUDY DESIGN: Animal study. SETTING: Experimental animal laboratory, tertiary referral center. SUBJECT AND METHOD: Twenty-four adult female Wistar Albino rats were grouped as: control (n = 6), reflux and irradiation (n = 10), and irradiation (n = 8). Rats were operated to create a reflux model 30 days before irradiation. Ionizing radiation was administered in a single fraction of a 20 Gy to the larynx. Laryngeal tissue samples were taken at the 4th day of irradiation and all specimens underwent histopathological examination. RESULTS:Edema and vascular dilation in lamina propria were higher in the reflux and irradiation, and irradiation groups than control group. Inflammation was higher in the reflux and irradiation group than the control group. Inflammation in squamous epithelium was higher in the reflux and irradiation and irradiation groups compared to the control group. Inflammation in the squamous epithelium of the irradiation group was higher than the reflux and irradiation group. In the respiratory tract epithelium, inflammation was higher in the reflux and irradiation group; additionally, a significant loss of cilia was present in the reflux and irradiation and irradiation groups while pseudostratification was higher in the reflux and irradiation group. CONCLUSION:Ionizing radiation-induced inflammation may increase on previously inflammated area due to gastroesophageal reflux. Therefore, it may be helpful to investigate and treat the reflux in laryngeal cancerpatients that will receive ionizing radiation.
Authors: J W Denham; L J Peters; J Johansen; M Poulsen; D S Lamb; A Hindley; P C O'Brien; N A Spry; M Penniment; H Krawitz; S Williamson; J Bear; L Tripcony Journal: Radiother Oncol Date: 1999-08 Impact factor: 6.280
Authors: Benjamin Saltman; Dennis H Kraus; Hazel Szeto; Bhupesh Parashar; Ronald Ghossein; Diane Felsen; Ryan C Branski Journal: Head Neck Date: 2010-05 Impact factor: 3.147