A multidimensional fractionation protocol for the oligomer analysis of oxidized waxes.

Oxidized waxes possess far superior properties as compared to the alkanes they are derived from. The separation of alkane oligomers via gas chromatography (GC) becomes a challenge when polar oxygen-containing functional groups are introduced or when higher molar masses are targeted. In the present study, the separation and analysis of oligomers in oxidized and non-oxidized waxes using different liquid chromatographic techniques are investigated. Oligomers in two oxidized waxes and a non-oxidized wax from which they are derived, are separated using high-temperature solvent gradient interaction chromatography (HT-SGIC) and high-temperature two-dimensional liquid chromatography (HT-2D-LC). Evaporative light scattering detector conditions are tailored to provide the best detection with the solvent system at use. It is shown that oligomers in oxidized and non-oxidized waxes can be separated and identified using the mentioned techniques. It has been found that the ELSD detector response systematically decreases as the oxidation levels of the waxes increase. Coupling of HT-HPLC and high-temperature size exclusion chromatography (HT-SEC) in a comprehensive 2D-LC setup shows a broadening of the molar mass distributions of the lower oligomer fractions as a consequence of the modification indicating changes in the oligomer chain microstructures. A preparative fractionation technique is utilized to collect specific oligomer fractions from the bulk waxes followed by hyphenation to HT-HPLC and other techniques. HPLC is shown to provide more detailed information on the oligomer composition of waxes when coupled to a pre-fractionation technique.