Literature DB >> 2986603

H+-gradient-dependent active transport of tetraethylammonium cation in apical-membrane vesicles isolated from kidney epithelial cell line LLC-PK1.

K Inui, H Saito, R Hori.   

Abstract

Transport of [3H]tetraethylammonium (NEt4+), an organic cation, has been studied by using apical-membrane vesicles isolated from cultured kidney epithelial cell line LLC-PK1. The uptake of NEt4+ by apical-membrane vesicles was osmotically sensitive, time-dependent and saturable. The presence of an H+ gradient ([H+]i greater than [H+]o) induced a marked stimulation of NEt4+ uptake against its concentration gradient (overshoot phenomenon), and this concentrative uptake was inhibited by HgCl2. These results suggest that apical membranes isolated from the LLC-PK1 cells retain the transport characteristics of NEt4+ similar to those observed in renal brush-border membranes.

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Year:  1985        PMID： 2986603      PMCID： PMC1144826          DOI： 10.1042/bj2270199

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  22 in total

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Authors:  B R Rennick
Journal:  Am J Physiol       Date:  1981-02

Review 2.  The use of isolated membrane vesicles to study epithelial transport processes.

Authors:  H Murer; R Kinne
Journal:  J Membr Biol       Date:  1980-07-15       Impact factor: 1.843

3.  Sugar transport in the LLC-PK1 renal epithelial cell line: similarity to mammalian kidney and the influence of cell density.

Authors:  J M Mullin; J Weibel; L Diamond; A Kleinzeller
Journal:  J Cell Physiol       Date:  1980-09       Impact factor: 6.384

4.  Transepithelial transport in cell culture: D-glucose transport by a pig kidney cell line (LLC-PK1).

Authors:  D S Misfeldt; M J Sanders
Journal:  J Membr Biol       Date:  1981-03-15       Impact factor: 1.843

5.  Na+-dependent hexose transport in vesicles from cultured renal epithelial cell line.

Authors:  A Moran; J S Handler; R J Turner
Journal:  Am J Physiol       Date:  1982-11

6.  Expression of a differentiated transport function in apical membrane vesicles isolated from an established kidney epithelial cell line. Sodium electrochemical potential-mediated active sugar transport.

Authors:  J E Lever
Journal:  J Biol Chem       Date:  1982-08-10       Impact factor: 5.157

7.  Polarized amino acid transport by an epithelial cell line of renal origin (LLC-PK1). The basolateral systems.

Authors:  C A Rabito; M V Karish
Journal:  J Biol Chem       Date:  1982-06-25       Impact factor: 5.157

8.  Localization of [3H]ouabain-sensitive Na+ pump sites in cultured pig kidney cells.

Authors:  J W Mills; A D Macknight; J M Dayer; D A Ausiello
Journal:  Am J Physiol       Date:  1979-03

9.  Na+-dependent sugar transport in a cultured epithelial cell line from pig kidney.

Authors:  C A Rabito; D A Ausiello
Journal:  J Membr Biol       Date:  1980       Impact factor: 1.843

10.  Interaction of ouabain with the Na+ pump in intact epithelial cells.

Authors:  J W Mills; A D Macknight; J A Jarrell; J M Dayer; D A Ausiello
Journal:  J Cell Biol       Date:  1981-03       Impact factor: 10.539
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  11 in total

1.  Kinetic analysis of tetraethylammonium transport in the kidney epithelial cell line, LLC-PK1.

Authors:  Y Tomita; Y Otsuki; Y Hashimoto; K Inui
Journal:  Pharm Res       Date:  1997-09       Impact factor: 4.200

2.  Molecular Comparison of Pyrophosphate- and ATP-Dependent Fructose 6-Phosphate 1-Phosphotransferases from Potato Tuber.

Authors:  N J Kruger; J B Hammond
Journal:  Plant Physiol       Date:  1988-03       Impact factor: 8.340

3.  Involvement of recognition and interaction of carnitine transporter in the decrease of L-carnitine concentration induced by pivalic acid and valproic acid.

Authors:  Noboru Okamura; Shuichi Ohnishi; Hiroyuki Shimaoka; Ryo Norikura; Hiroshi Hasegawa
Journal:  Pharm Res       Date:  2006-08       Impact factor: 4.200

4.  Mediation of cimetidine secretion by P-glycoprotein and a novel H(+)-coupled mechanism in cultured renal epithelial monolayers of LLC-PK1 cells.

Authors:  A J Dudley; C D Brown
Journal:  Br J Pharmacol       Date:  1996-03       Impact factor: 8.739

5.  Molecular cloning, functional characterization and tissue distribution of rat H+/organic cation antiporter MATE1.

Authors:  Tomohiro Terada; Satohiro Masuda; Jun-Ichi Asaka; Masahiro Tsuda; Toshiya Katsura; Ken-ichi Inui
Journal:  Pharm Res       Date:  2006-08       Impact factor: 4.200

6.  Decreased cellular toxicity of neomycin in a clonal cell line isolated from LLC-PK1.

Authors:  R Hori; M Okuda; Y Ohishi; M Yasuhara; K Inui; M Takano
Journal:  Pharm Res       Date:  1993-04       Impact factor: 4.200

7.  Expression of renal organic cation transporter in Xenopus laevis oocytes.

Authors:  R Hori; M Hirai; T Katsura; M Takano; M Yasuhara; S Kaneko; M Satoh
Journal:  Biochem J       Date:  1992-04-15       Impact factor: 3.857

8.  Cellular toxicity of aminoglycoside antibiotics in G418-sensitive and -resistant LLC-PK1 cells.

Authors:  M Takano; M Okuda; M Yasuhara; R Hori
Journal:  Pharm Res       Date:  1994-05       Impact factor: 4.200

9.  pH-dependent transport of procainamide in cultured renal epithelial monolayers of OK cells: consistent with nonionic diffusion.

Authors:  A J Dudley; C D Brown
Journal:  Br J Pharmacol       Date:  1995-09       Impact factor: 8.739

10.  Urinary Dopamine as a Potential Index of the Transport Activity of Multidrug and Toxin Extrusion in the Kidney.

Authors:  Moto Kajiwara; Tsuyoshi Ban; Kazuo Matsubara; Yoichi Nakanishi; Satohiro Masuda
Journal:  Int J Mol Sci       Date:  2016-07-30       Impact factor: 5.923
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