Zofia Bakuła1, Justyna Kościuch2, Aleksandra Safianowska3, Małgorzata Proboszcz4, Jacek Bielecki5, Jakko van Ingen6, Rafał Krenke7, Tomasz Jagielski8. 1. Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland. Electronic address: zofiabakula@biol.uw.edu.pl. 2. Department of Internal Medicine, Pulmonary Diseases & Allergy, Medical University of Warsaw, Warsaw, Poland. Electronic address: j_kosciuch@o2.pl. 3. Department of Internal Medicine, Pulmonary Diseases & Allergy, Medical University of Warsaw, Warsaw, Poland. Electronic address: aleksandra.safianowska@wum.edu.pl. 4. Department of Internal Medicine, Pulmonary Diseases & Allergy, Medical University of Warsaw, Warsaw, Poland. Electronic address: m.proboszcz@wp.pl. 5. Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland. Electronic address: jbielecki@biol.uw.edu.pl. 6. Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands. Electronic address: jakko.van.ingen@rivm.nl. 7. Department of Internal Medicine, Pulmonary Diseases & Allergy, Medical University of Warsaw, Warsaw, Poland. Electronic address: rafalkrenke@interia.pl. 8. Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland. Electronic address: t.jagielski@biol.uw.edu.pl.
Abstract
BACKGROUND: Studies concerning sociodemographic, clinical, and laboratory features of Mycobacterium kansasii pulmonary disease are few and based on small patient cohorts. The objective of the study was to evaluate characteristics of patients from whom M. kansasii respiratory isolates were recovered and to provide a detailed description of M. kansasii disease. BASIC PROCEDURES: Retrospective review of electronic medical records of all patients for whom at least one positive M. kansasii culture was obtained at the Department of Internal Medicine, Pulmonology and Allergology of the Warsaw Medical University between the year 2000 and 2015. Patients were categorized as having mycobacterial disease or as isolation cases based on the American Thoracic Society and Infectious Diseases Society of America (ATS/IDSA) diagnostic criteria. MAIN FINDINGS: The study comprised of 105 patients (63 females, 42 males, mean age 64.6 ± 17.8 years). Of these, 86 (81.9%) were diagnosed as having M. kansasii disease. The proportion of positive smear microscopy was significantly higher in patients with M. kansasii disease compared to M. kansasii isolation (P < 0.001). There were no statistically significant differences between M. kansasii disease and isolation cases in terms of clinical symptoms or comorbidities. Patients with M. kansasii disease presented most commonly (43/86, 50%) fibro-cavitary disease upon radiology. Lesion distribution usually showed bilateral upper lobe involvement. Among the 191 isolates genotyped, all were identified as M. kansasii type I. PRINCIPAL CONCLUSIONS: The findings from this study support the relaxation of the diagnostic criteria for the definition of M. kansasii disease, set forth by ATS/IDSA. Molecular typing did not differentiate isolates from patients with true disease from those with isolation only; the role of bacterial virulence factors thus remains elusive.
BACKGROUND: Studies concerning sociodemographic, clinical, and laboratory features of Mycobacterium kansasiipulmonary disease are few and based on small patient cohorts. The objective of the study was to evaluate characteristics of patients from whom M. kansasii respiratory isolates were recovered and to provide a detailed description of M. kansasii disease. BASIC PROCEDURES: Retrospective review of electronic medical records of all patients for whom at least one positive M. kansasii culture was obtained at the Department of Internal Medicine, Pulmonology and Allergology of the Warsaw Medical University between the year 2000 and 2015. Patients were categorized as having mycobacterial disease or as isolation cases based on the American Thoracic Society and Infectious Diseases Society of America (ATS/IDSA) diagnostic criteria. MAIN FINDINGS: The study comprised of 105 patients (63 females, 42 males, mean age 64.6 ± 17.8 years). Of these, 86 (81.9%) were diagnosed as having M. kansasii disease. The proportion of positive smear microscopy was significantly higher in patients with M. kansasii disease compared to M. kansasii isolation (P < 0.001). There were no statistically significant differences between M. kansasii disease and isolation cases in terms of clinical symptoms or comorbidities. Patients with M. kansasii disease presented most commonly (43/86, 50%) fibro-cavitary disease upon radiology. Lesion distribution usually showed bilateral upper lobe involvement. Among the 191 isolates genotyped, all were identified as M. kansasii type I. PRINCIPAL CONCLUSIONS: The findings from this study support the relaxation of the diagnostic criteria for the definition of M. kansasii disease, set forth by ATS/IDSA. Molecular typing did not differentiate isolates from patients with true disease from those with isolation only; the role of bacterial virulence factors thus remains elusive.
Authors: Laura Raniere Borges Dos Anjos; Poliana Lopes Parreira; Pedro Paulo Teixeira Silva Torres; André Kipnis; Ana Paula Junqueira-Kipnis; Marcelo Fouad Rabahi Journal: Rev Soc Bras Med Trop Date: 2020-09-11 Impact factor: 1.581
Authors: Tomasz Jagielski; Paulina Borówka; Zofia Bakuła; Jakub Lach; Błażej Marciniak; Anna Brzostek; Jarosław Dziadek; Mikołaj Dziurzyński; Lian Pennings; Jakko van Ingen; Manca Žolnir-Dovč; Dominik Strapagiel Journal: Front Microbiol Date: 2020-01-15 Impact factor: 5.640
Authors: Helena Modrá; Vít Ulmann; Jan Caha; Dana Hübelová; Ondřej Konečný; Jana Svobodová; Ross Tim Weston; Ivo Pavlík Journal: Int J Environ Res Public Health Date: 2019-10-17 Impact factor: 3.390