| Literature DB >> 2985639 |
Abstract
A simple procedure, suitable for routine epidemiological studies, is described for the differentiation of strains of cytomegaloviruses. Crude DNA from infected cells labelled with 32P was digested with restriction endonucleases and the resultant oligonucleotides were separated by electrophoresis on agarose gels. As few as 10(5) infected cells (i.e. a confluent monolayer of about 1 cm2) provided sufficient DNA for one analysis.Entities:
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Year: 1985 PMID: 2985639 DOI: 10.1016/0166-0934(85)90059-x
Source DB: PubMed Journal: J Virol Methods ISSN: 0166-0934 Impact factor: 2.014