| Literature DB >> 29856031 |
Gabriel Vachey1, Nicole Déglon2.
Abstract
This chapter describes the potential use of viral-mediated gene transfer in the central nervous system for genome editing in the context of Huntington's disease. Here, we provide protocols that cover the design of various genome editing strategies, the cloning of CRISPR/Cas9 elements into lentiviral vectors, and the assessment of cleavage efficiency, as well as potential unwanted effects.Entities:
Keywords: Brain; CRISPR; Cas9; Genome editing; Huntington’s disease; Lentiviral vector; Stereotaxic surgery
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Year: 2018 PMID： 29856031 DOI： 10.1007/978-1-4939-7825-0_21
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745