Rasa Liutkeviciene1,2, Alvita Vilkeviciute2, Alina Smalinskiene3, Abdonas Tamosiunas3, Janina Petkeviciene4, Dalia Zaliuniene1, Vaiva Lesauskaite3. 1. a Department of Ophthalmology , Lithuanian University of Health Sciences, Medical Academy , Kaunas , Lithuania. 2. b Neuroscience Institute , Lithuanian University of Health Sciences, Medical Academy , Kaunas , Lithuania. 3. c Institute of Cardiology , Medicine Academy, Lithuanian University of Health Sciences , Kaunas , Lithuania. 4. d Faculty of Public Health , Medical Academy, Lithuanian University of Health Sciences , Kaunas , Lithuania.
Abstract
BACKGROUND: Age-related macular degeneration (AMD) is the most common cause of incurable visual impairment in the developed countries. The main pathological change in AMD is the formation of drusen containing 40% of lipids, dominated by esterified cholesterol (EC) and phosphatidylcholine (PC), and protein. Haplotype ε4 of apolipoprotein E (ApoE) acts as a ligand for the low-density lipoprotein receptor and is involved in the maintenance and repair of neuronal cell membranes. PURPOSE: This study aimed to evaluate the association of AMD with ApoE gene polymorphism variants (rs7412 and rs429358). METHODOLOGY: A total of 2133 subjects were enrolled in our research. The study group comprised patients with early AMD (n = 413) and exudative AMD (n = 307), and the control group enrolled randomly selected persons (n = 1413). The genotyping of ApoE (rs7412 and rs429358) was performed using the real-time polymerase chain reaction (PCR) method. RESULTS: Statistical analysis revealed that ApoE 4/2 genotype was less frequently observed in in older patients with exudative AMD compared to older healthy controls (0.4% vs. 4.0%, p = 0.003). CONCLUSION: Our data demonstrated that ApoE 4/2 genotype was less frequently observed in old patients (65 years and more) with exudative AMD compared to old healthy controls. It leads to hypothesis on the protective effect of ApoE 4/2 to develop AMD in the elderly.
BACKGROUND: Age-related macular degeneration (AMD) is the most common cause of incurable visual impairment in the developed countries. The main pathological change in AMD is the formation of drusen containing 40% of lipids, dominated by esterified cholesterol (EC) and phosphatidylcholine (PC), and protein. Haplotype ε4 of apolipoprotein E (ApoE) acts as a ligand for the low-density lipoprotein receptor and is involved in the maintenance and repair of neuronal cell membranes. PURPOSE: This study aimed to evaluate the association of AMD with ApoE gene polymorphism variants (rs7412 and rs429358). METHODOLOGY: A total of 2133 subjects were enrolled in our research. The study group comprised patients with early AMD (n = 413) and exudative AMD (n = 307), and the control group enrolled randomly selected persons (n = 1413). The genotyping of ApoE (rs7412 and rs429358) was performed using the real-time polymerase chain reaction (PCR) method. RESULTS: Statistical analysis revealed that ApoE 4/2 genotype was less frequently observed in in older patients with exudative AMD compared to older healthy controls (0.4% vs. 4.0%, p = 0.003). CONCLUSION: Our data demonstrated that ApoE 4/2 genotype was less frequently observed in old patients (65 years and more) with exudative AMD compared to old healthy controls. It leads to hypothesis on the protective effect of ApoE 4/2 to develop AMD in the elderly.
Authors: Sarah C Conner; Laurent Benayoun; Jayandra J Himali; Stephanie L Adams; Qiong Yang; Charles DeCarli; Jan K Blusztajn; Alexa Beiser; Sudha Seshadri; Ivana Delalle Journal: J Alzheimers Dis Date: 2019 Impact factor: 4.472
Authors: Marina Gm Viturino; Jamil M Neto; Flávia F Bajano; Sueli Ms Costa; Alicia B Roque; Gessica Fs Borges; Galina Ananina; Priscila Hh Rim; Flávio M Medina; Fernando F Costa; José Pc de Vasconcellos; Mônica B de Melo Journal: Exp Biol Med (Maywood) Date: 2021-01-19