Rasmus Stenlid1,2, Hannes Manell1,2, Maria Halldin2, Joel Kullberg3,4, Håkan Ahlström3,4, Levon Manukyan1, Daniel Weghuber5,6, Katharina Paulmichl5,6, Fanni Zsoldos5,6, Peter Bergsten1,2, Anders Forslund2. 1. Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden. 2. Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden. 3. Department of Surgical Sciences, Section of Radiology, Uppsala University, Uppsala, Sweden. 4. Antaros Medical, Mölndal, Sweden. 5. Department of Pediatrics, Paracelsus Medical University, Salzburg, Austria. 6. Obesity Research Unit, Paracelsus Medical University, Salzburg, Austria.
Abstract
Context: Dipeptidyl peptidase 4 (DPP-4) metabolizes glucagon-like peptide-1 (GLP-1), and increased DPP4 levels are associated with obesity and visceral adiposity in adults. Objective: Investigating DPP-4 levels in adolescents and their association with (1) circulating intact GLP-1 levels and glucose tolerance; (2) body mass index (BMI); and (3) visceral, subcutaneous, and liver fat compartments. Design: Cross-sectional study, July 2012 to April 2015. Setting: Pediatric obesity clinic, Uppsala University Hospital. Patients and Participants: Children and adolescents with obesity (n = 59) and lean controls (n = 21) aged 8 to 18 years. Main Outcome Measures: BMI SD score, fasting plasma concentrations of DPP-4, total and intact GLP-1, fasting and oral glucose tolerance test (OGTT) concentrations of glucose, and visceral adipose tissue (VAT) and subcutaneous adipose tissue volumes and liver fat fraction. Results: Plasma DPP-4 levels decreased with age in both obese (41 ng/mL per year) and lean subjects (48 ng/mL per year). Plasma DPP-4 levels were higher in males in both the obesity and lean groups. With adjustments for age and sex, plasma DPP-4 level was negatively associated with intact GLP-1 at fasting (β = -12.3; 95% CI: -22.9, -1.8) and during OGTT (β = -12.1; 95% CI: -22.5, -1.7). No associations were found between DPP-4 and plasma glucose levels measured at fasting or after a 2-hour OGTT. Plasma DPP-4 level was 19% higher in obese subjects. Among adipose tissue compartments, the strongest association was with VAT (β = 0.05; 95% CI: -0.02, 0.12). Conclusions: In adolescents, high plasma DPP-4 concentrations were associated with low proportions of intact GLP-1, high BMI, young age, and male sex. The observed associations are compatible with increased metabolism of GLP-1 in childhood obesity.
Context:Dipeptidyl peptidase 4 (DPP-4) metabolizes glucagon-like peptide-1 (GLP-1), and increased DPP4 levels are associated with obesity and visceral adiposity in adults. Objective: Investigating DPP-4 levels in adolescents and their association with (1) circulating intact GLP-1 levels and glucose tolerance; (2) body mass index (BMI); and (3) visceral, subcutaneous, and liver fat compartments. Design: Cross-sectional study, July 2012 to April 2015. Setting: Pediatric obesity clinic, Uppsala University Hospital. Patients and Participants: Children and adolescents with obesity (n = 59) and lean controls (n = 21) aged 8 to 18 years. Main Outcome Measures: BMI SD score, fasting plasma concentrations of DPP-4, total and intact GLP-1, fasting and oral glucose tolerance test (OGTT) concentrations of glucose, and visceral adipose tissue (VAT) and subcutaneous adipose tissue volumes and liver fat fraction. Results: Plasma DPP-4 levels decreased with age in both obese (41 ng/mL per year) and lean subjects (48 ng/mL per year). Plasma DPP-4 levels were higher in males in both the obesity and lean groups. With adjustments for age and sex, plasma DPP-4 level was negatively associated with intact GLP-1 at fasting (β = -12.3; 95% CI: -22.9, -1.8) and during OGTT (β = -12.1; 95% CI: -22.5, -1.7). No associations were found between DPP-4 and plasma glucose levels measured at fasting or after a 2-hour OGTT. Plasma DPP-4 level was 19% higher in obese subjects. Among adipose tissue compartments, the strongest association was with VAT (β = 0.05; 95% CI: -0.02, 0.12). Conclusions: In adolescents, high plasma DPP-4 concentrations were associated with low proportions of intact GLP-1, high BMI, young age, and male sex. The observed associations are compatible with increased metabolism of GLP-1 in childhood obesity.
Authors: Rasmus Stenlid; David Olsson; Jing Cen; Hannes Manell; Charlotte Haglind; Azazul Islam Chowdhury; Peter Bergsten; Anna Nordenström; Maria Halldin Journal: Clin Transl Sci Date: 2021-08-26 Impact factor: 4.689
Authors: Khaled R Alkharsah; Salma Ali Aljaroodi; Jawad Ur Rahman; Awatif N Alnafie; Reem Al Dossary; Reem Y Aljindan; Amani M Alnimr; Jamal Hussen Journal: PLoS One Date: 2022-04-12 Impact factor: 3.240