Antoine Legras1,2, Anne Tallet3, Audrey Didelot2, Aurélie Cazes4,5, Claire Danel4, Angela Hin5, Raphaël Borie6, Bruno Crestani5,6, Yves Castier5,7, Patrick Bagan1,8, Françoise Le Pimpec-Barthes1, Marc Riquet1, Hélène Blons2,3, Pierre Mordant5,7. 1. Division of Thoracic Surgery and Lung Transplantation, Georges Pompidou European Hospital, Paris-Descartes University, Sorbonne Paris Cité University, Paris, France. 2. INSERM UMR-S1147, CNRS SNC 5014, Paris-Descartes University, Sorbonne Paris Cité University, Paris, France. 3. Division of Molecular Biology, Georges Pompidou European Hospital, Paris-Descartes University, Sorbonne Paris Cité University, Paris, France. 4. Division of Pathology, Bichat Hospital, Paris-Diderot University, Paris, France. 5. INSERM UMR-S1152, Bichat Hospital, Paris-Diderot University, Paris, France. 6. Division of Pneumology, Bichat Hospital, Paris-Diderot University, Paris, France. 7. Division of Thoracic and Vascular surgery, Bichat Hospital, Paris-Diderot University, Paris, France. 8. Division of Thoracic and Vascular surgery, Victor Dupouy Hospital, Argenteuil, France.
Abstract
BACKGROUND: Unicentric mediastinal Castleman disease (CD) is a rare condition, poorly characterized due to the small number of cases and the absence of genomic study. We analyzed clinical, radiological, histological and genomic patterns associated with mediastinal CD in a substantial case series. Methods: We retrospectively reviewed cases of unicentric mediastinal CD managed in 2 French thoracic surgery departments between 1988 and 2012. Clinical, radiological, surgical and pathological data were recorded. On available FFPE blocks we performed mutation screening by next-generation-sequencing, using AmpliSeq™ Cancer Hotspot v2 (Life Technologies) and immunohistochemistry (IHC) (AKT-mTOR pathway). RESULTS: Eleven patients were identified (mean age 41±15 years, sex-ratio 0.8, median follow-up 78 months). Surgical approach was thoracotomy (n=6), sternotomy (n=4), and VATS (n=1). Additional procedures included thymectomy in three cases, mediastinal lymphadenectomy in two cases, and bilobectomy in one case. One patient presented local relapse as a follicular dendritic cell sarcoma, leading to death 48 months after the first resection. Within 9 patients whose FFPE blocks were available, 2 mutations were found: VHL (p.F119L, 35%, n=1) and JAK3 (p.V718L, 53%, n=1). Phospho-AKT and phospho-mTOR stainings were negative in all cases, whereas phospho-S6RP staining was positive in eight cases, mainly in interfollicular cell cytoplasm. CONCLUSIONS: From this series of patients with unicentric mediastinal CD, we observed 2 cases of potential driver mutations and 8 cases of phospho-S6RP activation not related to AKT-mTOR. Larger studies are required to decipher more precisely the molecular abnormalities and potential therapeutic targets underlying this uncommon condition.
BACKGROUND: Unicentric mediastinal Castleman disease (CD) is a rare condition, poorly characterized due to the small number of cases and the absence of genomic study. We analyzed clinical, radiological, histological and genomic patterns associated with mediastinal CD in a substantial case series. Methods: We retrospectively reviewed cases of unicentric mediastinal CD managed in 2 French thoracic surgery departments between 1988 and 2012. Clinical, radiological, surgical and pathological data were recorded. On available FFPE blocks we performed mutation screening by next-generation-sequencing, using AmpliSeq™ Cancer Hotspot v2 (Life Technologies) and immunohistochemistry (IHC) (AKT-mTOR pathway). RESULTS: Eleven patients were identified (mean age 41±15 years, sex-ratio 0.8, median follow-up 78 months). Surgical approach was thoracotomy (n=6), sternotomy (n=4), and VATS (n=1). Additional procedures included thymectomy in three cases, mediastinal lymphadenectomy in two cases, and bilobectomy in one case. One patient presented local relapse as a follicular dendritic cell sarcoma, leading to death 48 months after the first resection. Within 9 patients whose FFPE blocks were available, 2 mutations were found: VHL (p.F119L, 35%, n=1) and JAK3 (p.V718L, 53%, n=1). Phospho-AKT and phospho-mTOR stainings were negative in all cases, whereas phospho-S6RP staining was positive in eight cases, mainly in interfollicular cell cytoplasm. CONCLUSIONS: From this series of patients with unicentric mediastinal CD, we observed 2 cases of potential driver mutations and 8 cases of phospho-S6RP activation not related to AKT-mTOR. Larger studies are required to decipher more precisely the molecular abnormalities and potential therapeutic targets underlying this uncommon condition.
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