Markus K Venäläinen1,2, Antti N Roine3, Merja R Häkkinen4, Jouko J Vepsäläinen4, Pekka S Kumpulainen5, Mikko S Kiviniemi6, Terho Lehtimäki7,8, Niku K Oksala8,9,10, Tuomo K Rantanen11,2,12. 1. Department of Surgery, School of Medicine, University of Eastern Finland, Kuopio, Finland. 2. Department of Surgery, Kuopio University Hospital, Kuopio, Finland. 3. Department of Surgery, Hatanpää Hospital, Tampere, Finland. 4. School of Pharmacy, University of Eastern Finland, Kuopio, Finland. 5. Department of Automation Science and Engineering, University of Technology, Tampere, Finland. 6. Department of Gastroenterology, Kuopio University Hospital, Kuopio, Finland. 7. Department of Clinical Chemistry, Fimlab Laboratories, Tampere, Finland. 8. Finnish Cardiovascular Research Center Tampere, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland. 9. Department of Surgery, Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland. 10. Department of Vascular Surgery, Tampere University Hospital, Tampere, Finland. 11. Department of Surgery, School of Medicine, University of Eastern Finland, Kuopio, Finland tuomora@gmail.com. 12. Seinäjoki Central Hospital, Seinäjoki, Finland.
Abstract
BACKGROUND: The declining mortality rate of patients with colorectal cancer (CRC) can be explained, at least partially, with early diagnosis. Simple diagnostic methods are needed to achieve a maximal patient participation rate in screening. MATERIALS AND METHODS: Liquid chromatography electrospray tandem mass spectrometry (LC-MS/MS) was used to determine urinary polyamine (PA) profiles. In a prospective setting, 116 patients were included in the study: 57 with CRC, 13 with inflammatory bowel disease (IBD), 12 with adenoma, and 34 controls. RESULTS: N1,N12-diacetylspermine (DiAcSPM) level was significantly higher in patients with CRC than controls (sensitivity=78.0%, specificity=70.6%; p=0.00049). The level of diacetylated cadaverine (p=0.0068) was lower and that of diacetylated putrescine (p=0.0078) was higher in patients with CRC than in those with IBD. Cadaverine (p=0.00010) and spermine (p=0.042) levels were lower and that of DiAcSPM (p=0.018) higher in patients with CRC than in those with adenoma. CONCLUSION: The simultaneous determination of urinary PAs by means of LC-MS/MS can be used to discriminate CRC from controls and patients with benign colorectal diseases. Copyright
BACKGROUND: The declining mortality rate of patients with colorectal cancer (CRC) can be explained, at least partially, with early diagnosis. Simple diagnostic methods are needed to achieve a maximal patient participation rate in screening. MATERIALS AND METHODS: Liquid chromatography electrospray tandem mass spectrometry (LC-MS/MS) was used to determine urinary polyamine (PA) profiles. In a prospective setting, 116 patients were included in the study: 57 with CRC, 13 with inflammatory bowel disease (IBD), 12 with adenoma, and 34 controls. RESULTS:N1,N12-diacetylspermine (DiAcSPM) level was significantly higher in patients with CRC than controls (sensitivity=78.0%, specificity=70.6%; p=0.00049). The level of diacetylated cadaverine (p=0.0068) was lower and that of diacetylated putrescine (p=0.0078) was higher in patients with CRC than in those with IBD. Cadaverine (p=0.00010) and spermine (p=0.042) levels were lower and that of DiAcSPM (p=0.018) higher in patients with CRC than in those with adenoma. CONCLUSION: The simultaneous determination of urinary PAs by means of LC-MS/MS can be used to discriminate CRC from controls and patients with benign colorectal diseases. Copyright
Authors: Lu Deng; Kathleen Ismond; Zhengjun Liu; Jeremy Constable; Haili Wang; Olusegun I Alatise; Martin R Weiser; T P Kingham; David Chang Journal: Cancer Epidemiol Biomarkers Prev Date: 2019-05-31 Impact factor: 4.254
Authors: Samuli I Nissinen; Markus Venäläinen; Pekka Kumpulainen; Antti Roine; Merja R Häkkinen; Jouko Vepsäläinen; Niku Oksala; Tuomo Rantanen Journal: Cancer Control Date: 2021 Jan-Dec Impact factor: 3.302