Julien Fernandes1,2,3,4, Fatima Hamidi1,2,3,4, Remi Leborgne5, Remi Beau6, Yves Castier1,2,3,4,7, Pierre Mordant1,2,3,4,7, Amira Boukkerou1,2,3,4, Jean Paul Latgé6, Marina Pretolani1,2,3,4. 1. INSERM UMR1152, Physiopathologie et Epidémiologie des Maladies Respiratoires, Paris, France. 2. Université Paris Diderot, Sorbonne Paris-Cité, Faculté de Médecine, site Bichat, France. 3. Laboratory of Excellence INFLAMEX, Université Sorbonne Paris-Cité, France. 4. Département Hospitalo-Universitaire FIRE, Paris, France. 5. ImagoSeine Electron Microscopy Facility, Institut Jacques Monod, Centre National de la Recherche Scientifique UMR 7592, Universite´ Paris Diderot, Sorbonne Paris-Cité, France. 6. Unité des Aspergillus, Institut Pasteur, Paris, France. 7. Service de Chirurgie Thoracique, Groupement Hospitalier Universitaire Nord Bichat-Claude Bernard, Assistance Publique des Hopitaux de Paris, France.
Abstract
Background: The airway epithelium is the first barrier interacting with Aspergillus fumigatus conidia after their inhalation, suggesting that this structure functions as point of entry of this fungus to initiate pulmonary aspergillosis. Methods: To study epithelial entry by A fumigatus, primary human reconstituted pseudostratified epithelium cultured in air-liquid interface as well as bronchial epithelial cell monolayers were infected with conidia. Results: Under these experimental conditions, we found that A fumigatus hyphae traversed the bronchial epithelium through a mechanism involving the recruitment of actin, which formed a tunnel that allows hyphae to enter the cells without disturbing their integrity. Conclusions: These findings describe a new mechanism by which A fumigatus hyphae penetrate the airway epithelial barrier and can infect its human host.
Background: The airway epithelium is the first barrier interacting with Aspergillus fumigatus conidia after their inhalation, suggesting that this structure functions as point of entry of this fungus to initiate pulmonary aspergillosis. Methods: To study epithelial entry by A fumigatus, primary human reconstituted pseudostratified epithelium cultured in air-liquid interface as well as bronchial epithelial cell monolayers were infected with conidia. Results: Under these experimental conditions, we found that A fumigatus hyphae traversed the bronchial epithelium through a mechanism involving the recruitment of actin, which formed a tunnel that allows hyphae to enter the cells without disturbing their integrity. Conclusions: These findings describe a new mechanism by which A fumigatus hyphae penetrate the airway epithelial barrier and can infect its human host.
Authors: Sigrid Bülow; Robert Heyd; Martina Toelge; Katharina U Ederer; Annette Schweda; Stefan H Blaas; Okka W Hamer; Andreas Hiergeist; Jürgen J Wenzel; André Gessner Journal: J Fungi (Basel) Date: 2020-11-20
Authors: Constanze Seidel; Sergio D Moreno-Velásquez; Nagwa Ben-Ghazzi; Sara Gago; Nick D Read; Paul Bowyer Journal: Front Microbiol Date: 2020-08-20 Impact factor: 5.640