Abdelaziz Ed-Dra1, Bouchra Karraouan2, Abdellah El Allaoui3, Meriem Khayatti2, Hicham El Ossmani4, Fouzia Rhazi Filali3, Naima ElMdaghri2, Brahim Bouchrif2. 1. Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Moulay Ismail University Faculty of Science, BP 11201 Zitoune Meknes, Morocco. Electronic address: abdelaziz_iaa@yahoo.fr. 2. Laboratory of Microbiology and Hygiene of Food and Water, Institut Pasteur du Maroc, 1 place Louis Pasteur, Casablanca 20100, Morocco. 3. Team of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Moulay Ismail University Faculty of Science, BP 11201 Zitoune Meknes, Morocco. 4. Genetics Laboratory of the Royal Gendarmerie, Ibn Sina Street, Rabat, Morocco.
Abstract
OBJECTIVES: Genotyping of Salmonella strains is an important molecular tool to discriminate isolates and to improve epidemiological studies when an outbreak occurs. Among the DNA-based genotyping methods, pulsed-field gel electrophoresis (PFGE) is currently used to subtype Salmonella isolates. In this study, the feasibility of genotyping Salmonella enterica serotype Infantis strains using XbaI restriction enzyme was evaluated. Separation of restricted fragments was performed by PFGE. METHODS: To test the possibility of applying this methodology to epidemiological investigation, a collection of 26 Salmonella Infantis strains were tested for their susceptibility to 14 antimicrobial agents and were analysed by XbaI macrorestriction followed by PFGE. Detection of class 1 integrons as well as intI1 and blaTEM genes in resistant strains was also studied. RESULTS: Antimicrobial susceptibility testing showed that 84.6% (22/26) of Salmonella Infantis isolates were susceptible to all of the antimicrobials tested, whereas 7.7% (2/26) had low-level resistance to β-lactams and harboured the blaTEM gene. A class 1 integron (0.8kb) and the intI1 gene (898bp) were detected in one Salmonella Infantis strain. However, five different PFGE profiles were defined by XbaI macrorestriction. CONCLUSIONS: The PFGE method demonstrated adequate typing ability and represents a powerful tool to discriminate the serotype Salmonella Infantis.
OBJECTIVES: Genotyping of Salmonella strains is an important molecular tool to discriminate isolates and to improve epidemiological studies when an outbreak occurs. Among the DNA-based genotyping methods, pulsed-field gel electrophoresis (PFGE) is currently used to subtype Salmonella isolates. In this study, the feasibility of genotyping Salmonella enterica serotype Infantis strains using XbaI restriction enzyme was evaluated. Separation of restricted fragments was performed by PFGE. METHODS: To test the possibility of applying this methodology to epidemiological investigation, a collection of 26 Salmonella Infantis strains were tested for their susceptibility to 14 antimicrobial agents and were analysed by XbaI macrorestriction followed by PFGE. Detection of class 1 integrons as well as intI1 and blaTEM genes in resistant strains was also studied. RESULTS: Antimicrobial susceptibility testing showed that 84.6% (22/26) of Salmonella Infantis isolates were susceptible to all of the antimicrobials tested, whereas 7.7% (2/26) had low-level resistance to β-lactams and harboured the blaTEM gene. A class 1 integron (0.8kb) and the intI1 gene (898bp) were detected in one Salmonella Infantis strain. However, five different PFGE profiles were defined by XbaI macrorestriction. CONCLUSIONS: The PFGE method demonstrated adequate typing ability and represents a powerful tool to discriminate the serotype Salmonella Infantis.
Authors: Pernille Gymoese; Kristoffer Kiil; Mia Torpdahl; Mark T Østerlund; Gitte Sørensen; John E Olsen; Eva M Nielsen; Eva Litrup Journal: BMC Genomics Date: 2019-11-15 Impact factor: 3.969
Authors: Nure Alam Siddiky; Samun Sarker; Shahidur Rahman Khan; Tanvir Rahman; Abdul Kafi; Mohammed A Samad Journal: PLoS One Date: 2022-02-07 Impact factor: 3.752
Authors: María Paula Herrera-Sánchez; Rafael Enrique Castro-Vargas; Luz Clemencia Fandiño-de-Rubio; Roy Rodríguez-Hernández; Iang Schroniltgen Rondón-Barragán Journal: Vet World Date: 2021-07-07