Literature DB >> 2984188

Viral Harvey ras p21 expressed in Escherichia coli purifies as a binary one-to-one complex with GDP.

M Poe, E M Scolnick, R B Stein.   

Abstract

The ras oncogene of Harvey murine sarcoma virus encodes a 21,000-dalton protein, p21, which mediates transformation. Viral Harvey ras p21, cloned into Escherichia coli HB101 lambda/pRAS1, has been purified to homogeneity by a three-step procedure. The purified E. coli p21 can be bound by a monoclonal antibody to viral Harvey ras p21 and has an amino acid composition consistent with that predicted from its DNA sequence. Purified E. coli p21 has been shown by HPLC analysis on an ion-exchange column to contain near stoichiometric amounts of GDP. This noncovalently associated GDP is seen in the UV absorption spectrum of the purified protein. The noncovalently associated GDP acts as a competitive inhibitor of the interaction of added guanine nucleotides with p21.

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Year:  1985        PMID： 2984188

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  19 in total

1.  Hypothermic stress leads to activation of Ras-Erk signaling.

Authors:  E Y Chan; S L Stang; D A Bottorff; J C Stone
Journal:  J Clin Invest       Date:  1999-05       Impact factor: 14.808

2.  p21 ras proteins and guanine nucleotides modulate the phosphorylation of 36- and 17-kilodalton mitochondria-associated proteins.

Authors:  J M Backer; I B Weinstein
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

3.  The arflike gene encodes an essential GTP-binding protein in Drosophila.

Authors:  J W Tamkun; R A Kahn; M Kissinger; B J Brizuela; C Rulka; M P Scott; J A Kennison
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

4.  Monoclonal antibody Y13-259 recognizes an epitope of the p21 ras molecule not directly involved in the GTP-binding activity of the protein.

Authors:  J C Lacal; S A Aaronson
Journal:  Mol Cell Biol       Date:  1986-04       Impact factor: 4.272

5.  ras p21 deletion mutants and monoclonal antibodies as tools for localization of regions relevant to p21 function.

Authors:  J C Lacal; S A Aaronson
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

Review 6.  The biochemistry of ras p21.

Authors:  R J Grand; D Owen
Journal:  Biochem J       Date:  1991-11-01       Impact factor: 3.857

7.  Glucagon and p21 ras enhance the phosphorylation of the same 38-kilodalton membrane protein from rat liver cells.

Authors:  A N Hegde; M R Das
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

8.  Guanine nucleotide binding properties of rap1 purified from human neutrophils.

Authors:  G M Bokoch; L A Quilliam
Journal:  Biochem J       Date:  1990-04-15       Impact factor: 3.857

9.  Biochemical and biological properties of the human N-ras p21 protein.

Authors:  M Trahey; R J Milley; G E Cole; M Innis; H Paterson; C J Marshall; A Hall; F McCormick
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

10.  The bovine papillomavirus E5 oncogene can cooperate with ras: identification of p21 amino acids critical for transformation by c-rasH but not v-rasH.

Authors:  B M Willumsen; W C Vass; T J Velu; A G Papageorge; J T Schiller; D R Lowy
Journal:  Mol Cell Biol       Date:  1991-12       Impact factor: 4.272
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