Literature DB >> 2983081

Purification of simian virus 40 large T antigen by immunoaffinity chromatography.

R A Dixon, D Nathans.   

Abstract

Simian virus 40 large T antigen from lytically infected cells has been purified to near homogeneity by immunochromatography of the cell extract on a protein A-Sepharose-monoclonal antibody column. The resulting T antigen retains biochemical activity; i.e., it hydrolyzes ATP and binds to simian virus 40 DNA at the origin of replication.

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Year:  1985        PMID: 2983081      PMCID: PMC254743     

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  10 in total

1.  Protein-DNA interactions at the origin of simian virus 40 DNA replication.

Authors:  R Tjian
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  Monoclonal antibodies specific for simian virus 40 tumor antigens.

Authors:  E Harlow; L V Crawford; D C Pim; N M Williamson
Journal:  J Virol       Date:  1981-09       Impact factor: 5.103

4.  Binding of a simian virus 40 T antigen-related protein to DNA.

Authors:  R D McKay
Journal:  J Mol Biol       Date:  1981-01-25       Impact factor: 5.469

5.  Catalytic properties of the SV40 large T antigen.

Authors:  R Tjian; A Robbins; R Clark
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1980

6.  A one-step purification of membrane proteins using a high efficiency immunomatrix.

Authors:  C Schneider; R A Newman; D R Sutherland; U Asser; M F Greaves
Journal:  J Biol Chem       Date:  1982-09-25       Impact factor: 5.157

7.  The use of thin acrylamide gels for DNA sequencing.

Authors:  F Sanger; A R Coulson
Journal:  FEBS Lett       Date:  1978-03-01       Impact factor: 4.124

8.  Simian virus 40 DNA replication in vitro.

Authors:  J J Li; T J Kelly
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

9.  Initiation of simian virus 40 DNA replication in vitro.

Authors:  H Ariga; S Sugano
Journal:  J Virol       Date:  1983-11       Impact factor: 5.103

10.  The isolation of simian virus 40 variants with specifically altered genomes.

Authors:  W W Brockman; D Nathans
Journal:  Proc Natl Acad Sci U S A       Date:  1974-03       Impact factor: 11.205

  10 in total
  39 in total

1.  Interactions required for binding of simian virus 40 T antigen to the viral origin and molecular modeling of initial assembly events.

Authors:  Danielle K Reese; Kodangattil R Sreekumar; Peter A Bullock
Journal:  J Virol       Date:  2004-03       Impact factor: 5.103

2.  ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.

Authors:  S P Deb; P Tegtmeyer
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103

3.  Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.

Authors:  K J Auborn; R B Markowitz; E Wang; Y T Yu; C Prives
Journal:  J Virol       Date:  1988-06       Impact factor: 5.103

4.  trans-Dominant and non-trans-dominant mutant simian virus 40 large T antigens show distinct responses to ATP.

Authors:  A M Castellino; P Cantalupo; I M Marks; J V Vartikar; K W Peden; J M Pipas
Journal:  J Virol       Date:  1997-10       Impact factor: 5.103

5.  Properties of the DNA-binding domain of the simian virus 40 large T antigen.

Authors:  D McVey; M Strauss; Y Gluzman
Journal:  Mol Cell Biol       Date:  1989-12       Impact factor: 4.272

6.  Removal of serine phosphates from simian virus 40 large T antigen increases its ability to stimulate DNA replication in vitro but has no effect on ATPase and DNA binding.

Authors:  F A Grässer; K Mann; G Walter
Journal:  J Virol       Date:  1987-11       Impact factor: 5.103

7.  Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences.

Authors:  A L DeLucia; S Deb; K Partin; P Tegtmeyer
Journal:  J Virol       Date:  1986-01       Impact factor: 5.103

8.  Functional organization of the simian virus 40 origin of DNA replication.

Authors:  J J Li; K W Peden; R A Dixon; T Kelly
Journal:  Mol Cell Biol       Date:  1986-04       Impact factor: 4.272

9.  Assembly of T-antigen double hexamers on the simian virus 40 core origin requires only a subset of the available binding sites.

Authors:  W S Joo; H Y Kim; J D Purviance; K R Sreekumar; P A Bullock
Journal:  Mol Cell Biol       Date:  1998-05       Impact factor: 4.272

10.  Hairpin structures are the primary amplification products: a novel mechanism for generation of inverted repeats during gene amplification.

Authors:  S Cohen; D Hassin; S Karby; S Lavi
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

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