The early appearance of fibronectin in the course of metastatic tumor growth in lymph nodes.

In order to study structural changes in the lymph node architecture in the course of metastatic involvement by carcinoma, 70 axillary lymph nodes of 45 female patients affected by breast carcinoma were examined. The following growth pattern of the metastasis was observed: (1) Involvement of vasa afferentia and (marginal) sinuses; (2) lysis of sinus wall and infiltration, causing replacement and lysis of reticulin fibres; (3) appearance of fibrillary fibronectin (FN) as the first noticeable sign of tumor stroma; (4) immigration of fibroblasts along these fibres, appearance of argyrophilic fibres codistributed with fibrillary FN; (5) formation of the complete tumor stroma, neovessels included. The appearance of FN is thus the first step of stroma formation and is interpreted as an "in situ" precipitation of soluble plasma FN.