Literature DB >> 2982841

The identification of histidine ligands to cytochrome a in cytochrome c oxidase.

C T Martin, C P Scholes, S I Chan.   

Abstract

A histidine auxotroph of Saccharomyces cerevisiae has been used to metabolically incorporate [1,3-15N2] histidine into yeast cytochrome c oxidase. Electron nuclear double resonance (ENDOR) spectroscopy of cytochrome a in the [15N]histidine-substituted enzyme reveals an ENDOR signal which can be assigned to hyperfine coupling of a histidine 15N with the low-spin heme, thereby unambiguously identifying histidine as an axial ligand to this cytochrome. Comparison of this result with similar ENDOR data obtained on two 15N-substituted bisimidazole model compounds, metmyoglobin-[15N]imidazole and bis[15N]imidazole tetraphenyl porphyrin, provides strong evidence for bisimidazole coordination in cytochrome a.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 2982841

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  6 in total

Review 1.  Cytochrome c oxidase metal centers: location and function.

Authors:  M Müller; A Azzi
Journal:  J Bioenerg Biomembr       Date:  1991-04       Impact factor: 2.945

2.  Binding of imidazole, 1-methylimidazole and 4-nitroimidazole to yeast cytochrome c peroxidase (CcP) and the distal histidine mutant, CcP(H52L).

Authors:  James E Erman; Diana Chinchilla; Jason Studer; Lidia B Vitello
Journal:  Biochim Biophys Acta       Date:  2015-04-20

3.  Redox-linked proton translocation in cytochrome oxidase: the importance of gating electron flow. The effects of slip in a model transducer.

Authors:  D F Blair; J Gelles; S I Chan
Journal:  Biophys J       Date:  1986-10       Impact factor: 4.033

4.  Cytochrome c oxidase from Paracoccus denitrificans: both hemes are located in subunit I.

Authors:  M Müller; B Schläpfer; A Azzi
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

5.  Definition of the catalytic site of cytochrome c oxidase: specific ligands of heme a and the heme a3-CuB center.

Authors:  J P Shapleigh; J P Hosler; M M Tecklenburg; Y Kim; G T Babcock; R B Gennis; S Ferguson-Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

6.  The role of copper and protons in heme-copper oxidases: kinetic study of an engineered heme-copper center in myoglobin.

Authors:  Jeffrey A Sigman; Hyeon K Kim; Xuan Zhao; James R Carey; Yi Lu
Journal:  Proc Natl Acad Sci U S A       Date:  2003-03-24       Impact factor: 11.205
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.