Maurizio Ronci1,2, Lidia Leporini3, Paolo Felaco4, Vittorio Sirolli4, Luisa Pieroni2, Viviana Greco2, Antonio Aceto1, Andrea Urbani2,5, Mario Bonomini4. 1. Department of Medical, Oral and Biotechnological Sciences, University "G. D'Annunzio" of Chieti-Pescara, Chieti 66100, Italy. 2. IRCCS-Santa Lucia Foundation, Rome 00144, Italy. 3. Department of Pharmacy, University "G. D'Annunzio" of Chieti-Pescara, Chieti 66100, Italy. 4. Department of Medicine and Aging Sciences, University "G. D'Annunzio" of Chieti-Pescara, Chieti 66100, Italy. 5. Institute of Biochemistry and Clinical Biochemistry, School of Medicine, Catholic University, Rome 00144, Italy.
Abstract
PURPOSE: The artificial membrane inside the haemodialyzer is the main determinant of the quality and success of haemodialysis therapy. The performances of haemodialysis membranes are highly influenced by the interactions with plasma proteins, which in turn are related to the physical and chemical characteristics of the membrane material. The present cross-over study is aimed to analyse the haemodialysis performance of a newly developed asymmetric cellulose triacetate membrane (ATA) in comparison to the conventional parent symmetric polymer (CTA). EXPERIMENTAL DESIGN: In four chronic non diabetic haemodialysis patients, the protein constituents of the adsorbed material from the filters after the haemodialysis session, and the proteins recovered in the ultrafiltrate during the session, are identified using a bottom-up shotgun proteomics approach. RESULTS: The ATA membrane shows a lower protein adsorption rate and a lower mass distribution pattern of the proteinaceous material. CONCLUSIONS AND CLINICAL RELEVANCE: By highlighting the differences between the two haemodialysis filters in terms of adsorbed proteins and flow through, it is demonstrated the higher biocompatibility of the novel ATA membrane, that fulfils the indications for the development of more performant membranes and may represent a step forward for the treatment of patients on chronic haemodialysis.
PURPOSE: The artificial membrane inside the haemodialyzer is the main determinant of the quality and success of haemodialysis therapy. The performances of haemodialysis membranes are highly influenced by the interactions with plasma proteins, which in turn are related to the physical and chemical characteristics of the membrane material. The present cross-over study is aimed to analyse the haemodialysis performance of a newly developed asymmetric cellulose triacetate membrane (ATA) in comparison to the conventional parent symmetric polymer (CTA). EXPERIMENTAL DESIGN: In four chronic non diabetic haemodialysispatients, the protein constituents of the adsorbed material from the filters after the haemodialysis session, and the proteins recovered in the ultrafiltrate during the session, are identified using a bottom-up shotgun proteomics approach. RESULTS: The ATA membrane shows a lower protein adsorption rate and a lower mass distribution pattern of the proteinaceous material. CONCLUSIONS AND CLINICAL RELEVANCE: By highlighting the differences between the two haemodialysis filters in terms of adsorbed proteins and flow through, it is demonstrated the higher biocompatibility of the novel ATA membrane, that fulfils the indications for the development of more performant membranes and may represent a step forward for the treatment of patients on chronic haemodialysis.
Authors: Gabriele Donati; Maria Cappuccilli; Chiara Donadei; Matteo Righini; Anna Scrivo; Lorenzo Gasperoni; Fulvia Zappulo; Gaetano La Manna Journal: Methods Protoc Date: 2021-04-22
Authors: Kamya Kameshwar; Matthew J Damasiewicz; Kevan R Polkinghorne; Peter G Kerr Journal: Nephrology (Carlton) Date: 2022-03-10 Impact factor: 2.358