X Roura1, G Santamarina2, M-D Tabar3, O Francino4, L Altet5. 1. Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola Del Vallès, 08193 Barcelona, Spain. Electronic address: xavier.roura@uab.cat. 2. Hospital Veterinario Universitario Rof Codina, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo, Spain. 3. Hospital Veterinario San Vicente, Calle Del Veterinario Manuel Isidro Rodríguez García N°17, San Vicente Del Raspeig, 03690 Alicante, Spain. 4. Molecular Genetics Veterinary Service, Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola Del Vallès, 08193 Barcelona, Spain. 5. Vetgenomics, Edifici Eureka, Parc de Recerca de La UAB, Bellaterra, Cerdanyola Del Vallès, 08193 Barcelona, Spain.
Abstract
OBJECTIVES: The presence of Bartonella spp. was detected by polymerase chain reaction (PCR) in dogs from Spain with blood culture-negative endocarditis. The aim of this study is to add information about canine infectious endocarditis in Europe. ANIMALS: Thirty dogs with naturally occurring blood culture-negative endocarditis were examined from 2010 to 2017 at three veterinary referral hospitals, located in northwest, northeast, and southeast of Spain. METHODS: It is a retrospective study. Medical records were reviewed to extract relevant data. Frozen or paraffin-embedded cardiac valve tissue and/or ethylenediamine tetraacetic acid blood samples were evaluated by PCR for the presence of Bartonella DNA. Positive results were sequenced to confirm the species. RESULTS: Polymerase chain reaction was positive for eight out of 30 dogs included (26.6%). Bartonella rochalimae, Bartonella vinsonii subsp. berkhoffii, and Bartonella koehlerae were detected in valve tissue or blood. CONCLUSIONS: Bartonella could be an important cause of blood culture-negative infectious endocarditis in dogs from Spain. The outcome for those dogs affected with Bartonella spp. was grave. Prompt empirical treatment with amoxicillin-clavulanate plus fluoroquinolones could be of value in cases of blood culture-negative endocarditis.
OBJECTIVES: The presence of Bartonella spp. was detected by polymerase chain reaction (PCR) in dogs from Spain with blood culture-negative endocarditis. The aim of this study is to add information about canineinfectious endocarditis in Europe. ANIMALS: Thirty dogs with naturally occurring blood culture-negative endocarditis were examined from 2010 to 2017 at three veterinary referral hospitals, located in northwest, northeast, and southeast of Spain. METHODS: It is a retrospective study. Medical records were reviewed to extract relevant data. Frozen or paraffin-embedded cardiac valve tissue and/or ethylenediamine tetraacetic acid blood samples were evaluated by PCR for the presence of Bartonella DNA. Positive results were sequenced to confirm the species. RESULTS: Polymerase chain reaction was positive for eight out of 30 dogs included (26.6%). Bartonella rochalimae, Bartonella vinsonii subsp. berkhoffii, and Bartonella koehlerae were detected in valve tissue or blood. CONCLUSIONS:Bartonella could be an important cause of blood culture-negative infectious endocarditis in dogs from Spain. The outcome for those dogs affected with Bartonella spp. was grave. Prompt empirical treatment with amoxicillin-clavulanate plus fluoroquinolones could be of value in cases of blood culture-negative endocarditis.
Authors: Erin Lashnits; Pradeep Neupane; Julie M Bradley; Toni Richardson; Rachael Thomas; Keith E Linder; Matthew Breen; Ricardo G Maggi; Edward B Breitschwerdt Journal: PLoS One Date: 2020-01-10 Impact factor: 3.240