Xin-Tong Xue1,2, Ting Zhang1,2, Sheng-Jie Cui1,2, Dan-Qing He1,2, Xue-Dong Wang1,2, Rui-Li Yang1,2, Da-Wei Liu1,2, Yan Liu1,2, Ye-Hua Gan3, Xiao-Xing Kou1,2, Yan-Heng Zhou1,2. 1. Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China. 2. Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Beijing, China. 3. Center for Temporomandibular Disorders and Orofacial Pain, Peking University School and Hospital of Stomatology, Beijing, China.
Abstract
OBJECTIVES: Temporomandibular joint osteoarthritis (TMJOA) is approximately twice as prevalent in women than in men. Synoviocytes are believed to play a critical role in joint inflammation. However, it is unknown whether synoviocytes from different genders possess sexual dimorphisms that contribute to female-predominant TMJOA. MATERIALS AND METHODS: Freund's complete adjuvant combined with monosodium iodoacetate was used to induce TMJOA in female and male rats. Histologic and radiographic features were used to evaluate TMJOA. The expression of CD68, MCP-1, iNOS, and IL-1β was detected by immunohistochemistry and real-time PCR. Primary fibroblast-like synoviocytes (FLSs) isolated from the synovial membrane of female and male rats were used for in vitro experiments. RESULTS: Female rats showed aggravated TMJOA features as compared to male rats. Increased expression of iNOS and IL-1β was detected in synovial membrane from female TMJOA rats as compared to male rats. Furthermore, greater amounts of CD68-positive macrophage infiltration and increased MCP-1 expression around the synovial membrane were detected in female TMJOA rats compared to males. Primary cultured FLSs from female rats showed higher sensitivity to TNF-α treatment and recruited increased macrophage migration than male FLSs. More important, ovariectomy (OVX) by ablation in female rats repressed the sensitivity of female FLSs to TNF-α treatment due to the loss of estrogen production. Blockage of the estrogen receptor repressed estrogen-potentiated TNF-α-induced pro-inflammatory cytokine expression in OVX-FLSs. Moreover, the injection of estrogen receptor antagonists relieved the cartilage destruction and bone deterioration of TMJOA in female rats. CONCLUSION: Estrogen-sensitized synoviocytes in female rats may contribute to gender differences in the incidence and progression of TMJOA.
OBJECTIVES:Temporomandibular joint osteoarthritis (TMJOA) is approximately twice as prevalent in women than in men. Synoviocytes are believed to play a critical role in joint inflammation. However, it is unknown whether synoviocytes from different genders possess sexual dimorphisms that contribute to female-predominant TMJOA. MATERIALS AND METHODS:Freund's complete adjuvant combined with monosodium iodoacetate was used to induce TMJOA in female and male rats. Histologic and radiographic features were used to evaluate TMJOA. The expression of CD68, MCP-1, iNOS, and IL-1β was detected by immunohistochemistry and real-time PCR. Primary fibroblast-like synoviocytes (FLSs) isolated from the synovial membrane of female and male rats were used for in vitro experiments. RESULTS: Female rats showed aggravated TMJOA features as compared to male rats. Increased expression of iNOS and IL-1β was detected in synovial membrane from female TMJOArats as compared to male rats. Furthermore, greater amounts of CD68-positive macrophage infiltration and increased MCP-1 expression around the synovial membrane were detected in female TMJOArats compared to males. Primary cultured FLSs from female rats showed higher sensitivity to TNF-α treatment and recruited increased macrophage migration than male FLSs. More important, ovariectomy (OVX) by ablation in female rats repressed the sensitivity of female FLSs to TNF-α treatment due to the loss of estrogen production. Blockage of the estrogen receptor repressed estrogen-potentiated TNF-α-induced pro-inflammatory cytokine expression in OVX-FLSs. Moreover, the injection of estrogen receptor antagonists relieved the cartilage destruction and bone deterioration of TMJOA in female rats. CONCLUSION: Estrogen-sensitized synoviocytes in female rats may contribute to gender differences in the incidence and progression of TMJOA.
Authors: Jennifer L Robinson; Pamela M Johnson; Karolina Kister; Michael T Yin; Jing Chen; Sunil Wadhwa Journal: Odontology Date: 2019-07-03 Impact factor: 2.634
Authors: Mario Rothbauer; Eva I Reihs; Anita Fischer; Reinhard Windhager; Florien Jenner; Stefan Toegel Journal: Front Bioeng Biotechnol Date: 2022-06-15
Authors: Natália Valdrighi; Juliana P Vago; Arjen B Blom; Fons A J van de Loo; Esmeralda N Blaney Davidson Journal: Front Pharmacol Date: 2022-05-19 Impact factor: 5.988
Authors: Sébastien Sannajust; Ian Imbert; Victoria Eaton; Terry Henderson; Lucy Liaw; Meghan May; Mary F Barbe; Tamara King Journal: Pain Date: 2019-09 Impact factor: 7.926
Authors: Rebecca S Hornung; William L Benton; Sirima Tongkhuya; Lynda Uphouse; Phillip R Kramer; Dayna Loyd Averitt Journal: Front Integr Neurosci Date: 2020-05-08