Guanglin Cui1, Aping Yuan2, Zhenglu Sun3, Wei Zheng3, Zhigang Pang4. 1. Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, China. Electronic address: guanglin.cui@yahoo.com. 2. Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, China; Laboratory of Molecular Cell Biology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway. 3. Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, China. 4. Research Group of Gastrointestinal Diseases, The Second Affiliated Hospital of Zhengzhou University, China; Department of General Surgery, The Second Affiliated Hospital of Zhengzhou University, China.
Abstract
OBJECTIVES: Recent studies suggest that the interaction between interleukin (IL)-1β and IL-6 in the microenvironment might be involved in the development and progression of human colorectal cancer (CRC). However, the expression of IL-1β/IL-6 network within the CRC microenvironment is not fully understood. MATERIALS AND METHODS: The level of IL-1β/IL-6 network expression in 40 biopsies of sporadic CRC and 15 biopsies of controls was assessed using quantitative real-time polymerase chain reaction (PCR) assay, immunohistochemistry (IHC) and double immunofluorescence staining. RESULTS: Quantitative results obtained by real-time PCR revealed that both IL-1β and IL-6 mRNA expressions were increased in CRC tissues compared with expressions in controls. In which, IL-6 mRNA expression in primary CRC tissues showed a statistically significant relationship with tumor invasion depth. IHC observations confirmed that increased expression of IL-1β and IL-6 immunoreactivities was located in both the CRC epithelium and stroma. Furthermore, IHC results also revealed that increased expression of IL-1β receptor type 1 (IL-1R1) and IL-6 receptor (IL-6R) were observed in both CRC epithelial and stromal cells. IHCs in serial CRC sections and double immunofluorescence staining revealed a highly co-expression of IL-1R1 immunoreactivity with IL-6 immunoreactivity in the same cells, which confirmed a histological fundament of IL-1β/IL-6 network. CONCLUSION: The IL-1β/IL-6 network is highly expressed in the CRC microenvironment, indicating that this network is important in the progression of CRC.
OBJECTIVES: Recent studies suggest that the interaction between interleukin (IL)-1β and IL-6 in the microenvironment might be involved in the development and progression of humancolorectal cancer (CRC). However, the expression of IL-1β/IL-6 network within the CRC microenvironment is not fully understood. MATERIALS AND METHODS: The level of IL-1β/IL-6 network expression in 40 biopsies of sporadic CRC and 15 biopsies of controls was assessed using quantitative real-time polymerase chain reaction (PCR) assay, immunohistochemistry (IHC) and double immunofluorescence staining. RESULTS: Quantitative results obtained by real-time PCR revealed that both IL-1β and IL-6 mRNA expressions were increased in CRC tissues compared with expressions in controls. In which, IL-6 mRNA expression in primary CRC tissues showed a statistically significant relationship with tumor invasion depth. IHC observations confirmed that increased expression of IL-1β and IL-6 immunoreactivities was located in both the CRC epithelium and stroma. Furthermore, IHC results also revealed that increased expression of IL-1β receptor type 1 (IL-1R1) and IL-6 receptor (IL-6R) were observed in both CRC epithelial and stromal cells. IHCs in serial CRC sections and double immunofluorescence staining revealed a highly co-expression of IL-1R1 immunoreactivity with IL-6 immunoreactivity in the same cells, which confirmed a histological fundament of IL-1β/IL-6 network. CONCLUSION: The IL-1β/IL-6 network is highly expressed in the CRC microenvironment, indicating that this network is important in the progression of CRC.
Authors: Su Yon Jung; Jeanette C Papp; Eric M Sobel; Matteo Pellegrini; Herbert Yu; Zuo-Feng Zhang Journal: Am J Cancer Res Date: 2020-09-01 Impact factor: 6.166
Authors: Yulia I Nussbaum; Yariswamy Manjunath; Kanve N Suvilesh; Wesley C Warren; Chi-Ren Shyu; Jussuf T Kaifi; Matthew A Ciorba; Jonathan B Mitchem Journal: Int J Mol Sci Date: 2021-04-30 Impact factor: 5.923