Oocyte-G1 promotes male germ cell apoptosis through activation of Caspase-3.

Apoptosis plays a vital role in the developmental process of the mammalian reproduction system, such as during folliculogenesis or spermatogenesis. Kinesin superfamily (Kif) proteins are responsible for intercellular transportation, and their malfunction can induce cell apoptosis. Oocyte-G1 is a new Kif member. Our previous study suggested that abnormal expression of Oocyte-G1 induced abnormal development of ovarian follicle and testes, but the underlying mechanism was not fully discovered. Therefore, in this study, the cellular role and mechanism of Oocyte-G1 were investigated. Transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) result showed that overexpression of Oocyte-G1 increased apoptosis in cultured cells. Oocyte-G1 transgenic mice also showed an increased apoptotic rate in male germ cells compared with controls. Immunoprecipitation and co-localization experiments revealed an interaction between Oocyte-G1 and Caspase-3. Expression levels of Caspase-3 were upregulated in cells overexpressing Oocyte-G1 and downregulated in Oocyte-G1 knockdown cells. These results suggest that Oocyte-G1 may promote male germ cell apoptosis through activating Caspase-3.