| Literature DB >> 29802926 |
Zhanchi Xu1, Xiaoteng Yan2, Zhuoyue Song1, Wei Li1, Weibo Zhao1, Huanhuan Ma1, Jiali Du1, Shijie Li3, Danyan Zhang4.
Abstract
Two novel heteropolysaccharides (JCH-1 and JCH-2) with molecular weights of 30.9 and 555.3 kDa were first extracted, isolated and purified from Isaria cicadae Miquel (I. Miquel). Monosaccharide analysis showed that JCH-1 and JCH-2 were composed of mannose, glucose and galactose with different monosaccharide ratio. In addition, JCH-1 had higher contents of sulfated and uronic acid compared to JCH-2. Based on MTT assay, JCH-1 and JCH-2 could markedly promote the proliferation of RAW264.7 cells and exhibit no cytotoxicity at a specific concentration range. The immunomodulatory assay exhibited that JCH-1 and JCH-2 could significantly enhance the viability of macrophage cells, and promote the release of NO, IL-6 and TNF-α. Furthermore, the immunomodulatory activity of JCH-1 was significantly better than that of JCH-2. These results proposed that I. Miquel had two polysaccharide fractions with different composition and JCH-1 is better to be developed as a functional food with the better immunomodulator activity.Entities:
Keywords: Immunomodulatory activity; Isaria cicadae Miquel; Polysaccharide
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Year: 2018 PMID： 29802926 DOI： 10.1016/j.ijbiomac.2018.05.164
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953