Dong Wei1,2, Weili Yu1, Chunyang Hu1, Lijuan Shen1, Tao Hu3. 1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Bei-Er-Jie Street, Haidian District, Beijing, 100190, China. 2. University of Chinese Academy of Sciences, Beijing, 100190, China. 3. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Bei-Er-Jie Street, Haidian District, Beijing, 100190, China. thu@home.ipe.ac.cn.
Abstract
OBJECTIVE: Capsular polysaccharide (PS) of Streptococcus pneumoniae is a key virulence factor and typically conjugated with a carrier protein. It is necessary to improve the immunogenicity of the conjugate vaccine against S. pneumoniae. RESULTS: A phenyl linker between tetanus toxoid (TT) and S. pneumoniae Type 14 PS was used to improve the PS-specific immunogenicity of the conjugate vaccine. As compared with the one with the amyl linker (PS-TT), the conjugate with the phenyl linker (PS-phe-TT) decreased the TT-specific IgG titers and significantly increased the PS-specific IgG titers and the IL-5 level. CONCLUSION: The phenyl linker could potentiate a robust humoral immune response to PS by decreasing the carrier-induced epitopic suppression effect. PS-phe-TT was expected to act as an effective vaccine against S. pneumoniae.
OBJECTIVE:Capsular polysaccharide (PS) of Streptococcus pneumoniae is a key virulence factor and typically conjugated with a carrier protein. It is necessary to improve the immunogenicity of the conjugate vaccine against S. pneumoniae. RESULTS: A phenyl linker between tetanus toxoid (TT) and S. pneumoniae Type 14 PS was used to improve the PS-specific immunogenicity of the conjugate vaccine. As compared with the one with the amyl linker (PS-TT), the conjugate with the phenyl linker (PS-phe-TT) decreased the TT-specific IgG titers and significantly increased the PS-specific IgG titers and the IL-5 level. CONCLUSION: The phenyl linker could potentiate a robust humoral immune response to PS by decreasing the carrier-induced epitopic suppression effect. PS-phe-TT was expected to act as an effective vaccine against S. pneumoniae.
Authors: Li Ou; Wing-Pui Kong; Gwo-Yu Chuang; Mridul Ghosh; Krishana Gulla; Sijy O'Dell; Joseph Varriale; Nathan Barefoot; Anita Changela; Cara W Chao; Cheng Cheng; Aliaksandr Druz; Rui Kong; Krisha McKee; Reda Rawi; Edward K Sarfo; Arne Schön; Andrew Shaddeau; Yaroslav Tsybovsky; Raffaello Verardi; Shuishu Wang; Timothy G Wanninger; Kai Xu; Gengcheng J Yang; Baoshan Zhang; Yaqiu Zhang; Tongqing Zhou; Frank J Arnold; Nicole A Doria-Rose; Q Paula Lei; Edward T Ryan; Willie F Vann; John R Mascola; Peter D Kwong Journal: Sci Rep Date: 2020-02-20 Impact factor: 4.996