Huijing Bao1, Kai Jiang2, Kai Meng3, Wenjing Liu4, Ping Liu4, Yunhong Du4, Dabo Wang5. 1. Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China; Department of Ophthalmology, Central Hospital of Taian, Taian, Shandong, 271000, China. 2. Department of Ophthalmology, Yuhuangding Hospital, Yantai, Shandong, 264001, China. 3. Department of Anorectal Surgery, Central Hospital of Taian, Taian, Shandong, 271000, China. 4. Department of Ophthalmology, Central Hospital of Taian, Taian, Shandong, 271000, China. 5. Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China. Electronic address: wangdabo354@163.com.
Abstract
AIM: The aim of this study was to research the effect of TGF-β2 on human enon capsule fibroblasts proliferation and apoptosis and its potential mechanism. METHODS: Human eyeball fascia tissues (n = 45) were derived from ocular fascia tissues of patients who were underwent glaucoma filtration surgery, and Tenon capsule fibroblasts were obtained from these tissues. Liposome-mediated transfection, CCK8 assay, Hoechst33258 staining, qRT-PCR detection, western blot, and luciferase reporter assay were performed. RESULTS: TGF-β2 promoted proliferation and inhibited apoptosis of human Tenon capsule fibroblasts in a dose-dependent manner. TGF-β2 induced down-regulation of miR-26 and up-regulation of CTGF in a dose-dependent manner. CTGF was the target gene of miR-26 and miR-26 had a negative regulatory effect on CTGF expression. miR-26 up-regulation could significantly decrease proliferation and increase apoptosis of human Tenon capsule fibroblasts after induced by TGF-β2 (P < 0.05). Down-regulation of CTGF could markly decrease proliferation and increase apoptosis of human Tenon capsule fibroblasts after induced by TGF-β2 (P < 0.05). CONCLUSION: miR-26 could inhibit proliferation and promote apoptosis of human Tenon capsule fibroblasts after they were induced by TGF-β2 through suppressing CTGF expression.
AIM: The aim of this study was to research the effect of TGF-β2 on human enon capsule fibroblasts proliferation and apoptosis and its potential mechanism. METHODS:Human eyeball fascia tissues (n = 45) were derived from ocular fascia tissues of patients who were underwent glaucoma filtration surgery, and Tenon capsule fibroblasts were obtained from these tissues. Liposome-mediated transfection, CCK8 assay, Hoechst33258 staining, qRT-PCR detection, western blot, and luciferase reporter assay were performed. RESULTS: TGF-β2 promoted proliferation and inhibited apoptosis of human Tenon capsule fibroblasts in a dose-dependent manner. TGF-β2 induced down-regulation of miR-26 and up-regulation of CTGF in a dose-dependent manner. CTGF was the target gene of miR-26 and miR-26 had a negative regulatory effect on CTGF expression. miR-26 up-regulation could significantly decrease proliferation and increase apoptosis of human Tenon capsule fibroblasts after induced by TGF-β2 (P < 0.05). Down-regulation of CTGF could markly decrease proliferation and increase apoptosis of human Tenon capsule fibroblasts after induced by TGF-β2 (P < 0.05). CONCLUSION:miR-26 could inhibit proliferation and promote apoptosis of human Tenon capsule fibroblasts after they were induced by TGF-β2 through suppressing CTGF expression.
Authors: Bin Wang; Aiqing Zhang; Haidong Wang; Janet D Klein; Lin Tan; Ze-Mu Wang; Jie Du; Nawazish Naqvi; Bi-Cheng Liu; Xiaonan H Wang Journal: Theranostics Date: 2019-03-07 Impact factor: 11.556