Postprandial Lipid Concentrations and Daytime Biological Variation of Lipids in a Healthy Chinese Population.

BACKGROUND: Several latest guidelines and consensus statements from Europe and the United States specify that there is no need for fasting prior to routine lipid tests. However, the latest Chinese guidelines still recommend fasting tests owing to a lack of local evidence. This study aimed to investigate postprandial lipid concentrations and daytime biological variation of lipids in a healthy Chinese population.
METHODS: Venous blood samples were collected from 41 ostensibly healthy Chinese volunteers at five time points during the day (06:30, 09:00, 12:00, 15:00, and 18:30). The same batch of reagents was used to determine lipid concentrations. A nested ANOVA was performed to calculate within-subject biological variation (CV(I)) and between-subject biological variation (CV(G)).
RESULTS: Postprandial concentrations of triglyceride were higher than fasting concentrations, with the maximum change occurring at 12:00 (0.5 hours after lunch, 0.21±0.65 mmol/L difference). The daytime biological variation of triglycerides was relatively high (CV(I)=25%, CV(G)=35.9%). The postprandial concentrations of total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, apolipoprotein A1, and apolipoprotein B were mostly lower than the fasting concentrations, and their daytime biological variations were relatively low (CV(I)=2.4-4.4%, CV(G)=11.8-18.7%).
CONCLUSIONS: As most daytime lipid concentrations changed only slightly, non-fasting samples could be used for routine lipid tests. However, in cases of abnormal postprandial triglyceride concentrations, dietary factors and fasting time should be considered when interpreting the results. © The Korean Society for Laboratory Medicine.

INTRODUCTION

Serum lipid profiling is a routine biochemical test used for cardiovascular risk prediction and early detection and diagnosis of hyperlipidemia; this test can also assist in the diagnosis of atherosclerosis [1]. Although individuals spend the vast majority of their time in the postprandial state, the 2016 Chinese guidelines for prevention and treatment of dyslipidemia for Chinese adults recommended at least 12 hrs of fasting prior to blood sampling in order to standardize the lipid tests [2]. A number of recent guidelines and consensus statements have suggested that fasting is not necessary prior to routine lipid tests, and that fasting lipid tests should be considered only when the triglyceride (TG) concentration is >5.0 mmol/L or in some specific clinical situations, such as the diagnosis of hypertriglyceridemia [3456]. Compared with fasting lipid tests, non-fasting lipid tests would simplify the blood-sampling process for both patients and hospitals.

Previous reports regarding changes in postprandial lipids originated mainly from the United States and Europe [356]. These studies have demonstrated that while the postprandial concentrations of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (apo-A1), and apolipoprotein B (apo-B) changed minimally, TG concentration exhibited an obvious increase after meals [78]. However, few studies have investigated the changes in postprandial lipid concentrations in Chinese populations. These studies focused on oral fat tolerance tests [91011], and no study has examined postprandial lipid concentrations after normal food intake [12].

Biological variation (BV) can be defined as a natural fluctuation of analytes caused by biological factors such as time period, population, and physiology. BV can be used for establishing analytical goals for the presence of bias and imprecision, evaluating the significance of time-course changes in an individual's lipid test results, and assessing the clinical utility of population-based reference intervals [13]. The current lipid BV data have been collected mainly from European and American populations [14151617]. Moreover, the majority of these studies were conducted 20 years ago, and the samples were mostly collected during a fasting state [141518]. Over the years, BV research and statistical analysis methods have been updated [1617]. Against this background, the present study aimed to provide clinical references for non-fasting lipid tests, by examining postprandial lipid concentrations and daytime lipid BV in a healthy Chinese population, while taking into account differences in ethnicity, environment, and lifestyle across populations, and on the basis of the latest BV research guidelines and standards [1319].

MATERIALS AND METHODS

1. Subjects

Forty-one ostensibly healthy volunteers (21 males and 20 females) of Han Chinese ethnicity participated in this study from March to April 2015. Their general characteristics are shown in Table 1. None reported any diagnosed chronic diseases or history of recent medications. Except for one male subject, all reported no history of smoking or alcohol consumption.

Table 1

General characteristics of the study subjects (N=41)

	Males (N = 21)	Female (N = 20)
Age (year)	25.4 ± 5.2	25.9 ± 7.2
Height (cm)	172.5 ± 68.5	159.1 ± 45.8
Weight (kg)	68.4 ± 12.3	51.7 ± 5.1
Body mass index	22.8 ± 3.0	20.5 ± 2.1

Data are presented as mean±standard deviation.

Before participation, the subjects were informed that they should not change their lifestyle and usual diet three days prior to and on the study day. Following study completion, the subjects were asked to confirm that they met these requirements. This study was approved by the Institutional Review Board of the First Affiliated Hospital, College of Medicine, Zhejiang University, China. All subjects signed informed consent forms prior to participation and were offered approximately 30 USD as compensation.

2. Sample size calculation

The confidence interval and test power for within-subject BV (CVI) were calculated based on the analytical precision, number of replicates, number of samples, and number of subjects [20]. Specifically, 41 subjects participated in the study, blood samples were collected from each subject five times, and each sample was tested in duplicate. Combined with the existing lipid analytical precision and CVI [18], the CVI test power was 100%, indicating that our sample size was sufficient.

3. Sample collection

Considering the convenience of blood sample collection for the subjects, five blood sampling time points were set throughout the day: 6:30 (fasting), 9:00 (2 hours after breakfast), 12:00 (0.5 hours after lunch), 15:00 (3.5 hours after lunch), and 18:30 (1 hour after dinner). The participants were instructed to follow the exact mealtimes (completing breakfast, lunch, and dinner by 7:00, 11:30, and 17:30, respectively); all subjects complied with these instructions. All blood samples were drawn by a single, experienced phlebotomist. Samples were collected from three to seven subjects per day. Prior to phlebotomy, the subjects were required to sit and rest for approximately 15 minutes. Three milliliters of blood was collected from each subject at each time point; samples were added to tubes containing a clot activator and gel serum separator. The tubes were inverted eight times for mixing purposes and placed at room temperature (22–25℃) for 0.5–1 hours to allow clot retraction and separation of serum. Next, the serum of the blood samples was obtained by centrifugation (4,000 r/minutes×6 minutes) and stored at −80℃.

4. Instruments and analytical methods

TG and TC were measured using an enzymatic colorimetric test method and the reagents for the tests were purchased from Roche Diagnostics (Roche, Basle, Switzerland). TG measurements were traceable to reference material SRM 909b, and TC measurements were traceable to the isotope dilution-mass spectrometry reference method. HDL-C and LDL-C were measured using homogeneous enzymatic colorimetric test methods and the reagents for the tests were purchased from Roche Diagnostics (Roche, Basle, Switzerland). HDL-C was measured using the 3rd generation Roche assay, traceable to the Centers for Disease Control (CDC) reference method. LDL-C was measured using the 2nd generation Roche assay, traceable to the CDC beta quantification method. Apo-A1 and apo-B were determined using an immunoturbidimetric method with reagents obtained from Shensuo Company (Youfu, Shanghai, China). Apo-A1 measurements were traceable to WHO international reference material SP1-01, and apo-B measurements were traceable to WHO international reference material SP3-07. LDL-C (calculated) was determined using the Friedewald equation:

LDL-C=TC−HDL-C−TG/2.2.

The frozen serum samples were thawed by keeping them at 22–25℃ for 3 hours. Following inversion and proper mixing, all serum samples were tested in duplicate on a Hitachi 7600 automated analyzer (Hitachi, Tokyo, Japan) with the same batch of regents.

5. Statistical analysis

Cochran's test was used to exclude the presence of outliers such as duplicate samples, individual variance, and mean values [13]. Normality tests for the lipid data were performed using the Shapiro-Wilk method. Changes in the lipid results of the five time points were analyzed with a one-way ANOVA. Postprandial and fasting lipid results were compared using a paired samples t-test. Results from male and female subjects were compared using the independent samples t-test and F test. Within-subject changes in lipid concentration at the five time points were illustrated using box plots. All the above analyses were conducted using SPSS 19.0 (SPSS Inc., Chicago, IL, USA). P<0.05 was considered statistically significant.

Based on the methods used by Braga and Panteghini [13], a nested random-effects ANOVA was performed using SAS 9.2 (SAS Institute Inc., Cary, NC, USA). The mean square of S2T (the total variance of all measurements), S2I+A (average within-subject total variance), and S2A, (average with-run analytical variance) were calculated. The S2I (average within-subject biological variance) was calculated using the formula:

S

S2G (between-subject biological variance) was calculated using the formula:

S

where k is the number of samples per subject, and r is the number of subjects. Finally, the analytical coefficient of variation (CVA), CVI, between-subject BV (CVG), index of individuality (II), and reference change value (RCV) were calculated based on the following formulae:

CV

and

RCV=2.77(CV

In addition, BV 95% confidence intervals (CIs) were calculated using Burdick et al's method [21].

RESULTS

1. Changes in postprandial lipid concentrations

According to the 2016 Chinese adult dyslipidemia prevention and treatment guidelines [1], there were six cases of abnormal TG concentrations, with a median of 2.66 mmol/L (range, 2.41–5.59 mmol/L; TG≥2.3 mmol/L is considered abnormal), one case of abnormal TC with 7.11 mmol/L (TC≥6.22 mmol/L is considered abnormal), seven cases of abnormal HDL-C with a median of 0.80 mmol/L (range, 0.69–0.95 mmol/L; HDL-C<1.0 mmol/L is considered abnormal), and one case of abnormal LDL-C with 4.73 mmol/L (LDL-C≥4.1 mmol/L is considered abnormal).

The lipid results are shown in Table 2. Data were found to be normally distributed. The changes in postprandial lipid concentrations were relatively small, and there were no differences between the five time points for any of the six lipid parameters (P>0.05). The postprandial TG concentrations were higher than the fasting concentrations, with the maximum change (0.21±0.65 mmol/L) occurring at 12:00 (0.5 hours postprandial). The postprandial concentrations of TC, HDL-C, LDL-C, apo-A1, and apo-B were mostly lower than the fasting concentrations, with relatively low rates of change (<−4%). Most lipid parameter concentrations at 9:00, 12:00, and 15:00 were different from fasting concentrations (P<0.05 or P<0.01). Table 3 shows a comparison between the maximum mean changes in fasting lipid and postprandial lipid concentrations identified in this study and those reported in previous studies. The changes in postprandial lipid concentration in our subjects were relatively smaller than the changes reported in previous studies.

Table 2

Postprandial lipid changes in the 41 study subjects

Analyte (Units)	6:30 (fasting)	9:00 (2 hours postprandial)	12:00 (0.5 hours postprandial)	15:00 (3.5 hours postprandial)	18:30 (1 hour postprandial)
Triglyceride (mmol/L)	1.28 ± 0.7	1.38 ± 0.68	1.49 ± 0.73∥	1.43 ± 0.74	1.41 ± 0.65
Concentration change* (mmol/L)	N/A	0.10 ± 0.52	0.21 ± 0.65	0.15 ± 0.53	0.13 ± 0.58
Change rate† (%)	N/A	14.2 ± 31.2	27.9 ± 56.4	20.8 ± 45.1	23.4 ± 55.3
Increased/decreased cases‡	N/A	29/12	26/15	24/17	29/12
P	N/A	0.219	0.048	0.080	0.165
Total cholesterol (mmol/L)	4.18 ± 0.73	4.09 ± 0.74¶	4.03 ± 0.7¶	4.07 ± 0.72∥	4.15 ± 0.76
Concentration change* (mmol/L)	N/A	−0.08 ± 0.18	−0.15 ± 0.23	−0.1 ± 0.31	−0.03 ± 0.25
Change rate† (%)	N/A	−2.0 ± 4.3	−3.3 ± 5.4	−2.2 ± 8.0	−0.8 ± 6.2
Increased/decreased cases‡	N/A	13/28	12/29	13/28	13/24
P	N/A	0.005	< 0.001	0.037	0.439
LDL-C (mmol/L)	2.35±0.62	2.28±0.61¶	2.24±0.62¶	2.28±0.61∥	2.32±0.69
Concentration change* (mmol/L)	N/A	−0.07±0.16	−0.11±0.18	−0.07±0.17	−0.02±0.22
Change rate† (%)	N/A	−2.9±9.1	−4.4±10.3	−2.6±8.7	−0.9±11.9
Increased/decreased cases‡	N/A	11/30	8/33	12/29	15/26
P	N/A	0.005	<0.001	0.012	0.534
LDL-C (calculated)§ (mmol/L)	2.39±0.59	2.32±0.60¶	2.23±0.62∥	2.29±0.55∥	2.33±0.63
Concentration change* (mmol/L)	N/A	−0.08±0.24	−0.17±0.33	−0.11±0.35	−0.07±0.35
Change rate† (%)	N/A	−3.0±10.4	−6.6±15.2	−3.7±10.6	−2.3±14.8
Increased/decreased cases‡	N/A	13/28	12/29	12/29	14/27
P	N/A	0.004	0.023	0.041	0.569
HDL-C (mmol/L)	1.19±0.28	1.15±0.25¶	1.14±0.25¶	1.15±0.29∥	1.19±0.27
Concentration change* (mmol/L)	N/A	−0.05±0.69	−0.05±0.08	−0.04±0.1	0.00±0.1
Change rate† (%)	N/A	−3.5±5.1	−4.0±7.3	−2.8±11.2	0.2±9.2
Increased/decreased cases‡	N/A	10/31	7/34	11/30	20/21
P	N/A	<0.001	<0.001	0.022	0.874
Apolipoprotein A1 (g/L)	1.62±0.22	1.59±0.23¶	1.58±0.22¶	1.60±0.23	1.62±0.23
Concentration change* (mmol/L)	N/A	−0.03±0.06	−0.04±0.07	−0.02±0.11	0.01±0.08
Change rate† (%)	N/A	−1.9±3.7	−2.3±3.9	−0.8±7.1	0.4±4.4
Increased/decreased cases‡	N/A	14/27	13/28	18/23	23/18
P	N/A	0.004	0.001	0.362	0.577
Apolipoprotein B (g/L)	0.68±0.14	0.66±0.13¶	0.66±0.14¶	0.67±0.14∥	0.68±0.15
Concentration change* (mmol/L)	N/A	−0.02±0.04	−0.03±0.04	−0.02±0.04	−0.01±0.04
Change rate† (%)	N/A	−3.4±5.1	−3.7±5.2	−2.1±5.6	−0.7±5.8
Increased/decreased cases‡	N/A	13/28	10/31	16/25	16/25
P	N/A	<0.001	<0.001	0.018	0.414

Data are presented as mean±standard deviation or number.

*Concentration change refers to the current postprandial lipid concentration minus the fasting concentration; †Change rate was calculated using the formula [(postprandial concentration minus fasting concentration)/fasting concentration]×100%; ‡Increased cases refer to subjects whose postprandial test results were higher than the fasting results, while decreased cases refer to subjects whose postprandial test results were lower than the fasting results; §LDL-C (calculated) was determined using the Friedewald equation: LDL-C=TC−HDL-C−TG/2.2; ∥P<0.05, ¶P<0.01, paired sample t-test.

Abbreviations: N/A, not available; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol.

Table 3

Comparison between the maximum mean changes in fasting lipid and postprandial lipid concentrations identified in the current study and in previous studies

Reference	Country and population	Diet	Sample size (N)	Fasting time (hour)	TG (mmol/L)	TC (mmol/L)	LDL-C (mmol/L)	HDL-C (mmol/L)	apo-A1 (g/L)	apo-B (g/L)
Current study	Ostensibly healthy Chinese volunteers	Typical Han Chinese	41	0.5–3.5	+0.21	−0.15	−0.11	−0.05	−0.04	−0.03
Mihas et al [8]*	Caucasian race	Fat tolerance test	3,749	4	+1.06	/	/	/	/	/
Langsted et al [22]	Denmark, general population	Normal food intake	26,613	0–4	+0.3	−0.2	−0.2	−0.1	−2.6	+2.6
Sidhu and Naugler [23]	Canada, community-based population	Not reported	209,180	1–16	+0.3	<2%†	−0.1	<2%†	/	/
Gruchot et al [24]	Germany, population without alcoholic fatty liver diseases	Not reported	257	1–4	+0.5	−0.1	−0.4	0.1	/	/
Aronov et al [25]	Russian men without coronary heart disease	Standard fat load	20	3	+1.6	−0.15	−0.21	−0.05	+0.18	−0.08

*This article is a meta-analysis; †The change is reported as “percent” in the original paper.

Abbreviations: TG, triglyceride; TC, total cholesterol; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol; apo-A1, apolipoprotein A1; apo-B, apolipoprotein B.

2. Within-subject changes in lipid concentrations

Lipid distribution at the five time points is shown in Fig. 1. The daytime changes in TG were the highest (Fig. 1A), while changes in the other lipid parameters were relatively small (Fig. 1B–1F). Moreover, within-subject daytime changes in the lipid concentrations varied widely. In addition, many outliers and extremes were observed in the results of each lipid parameter.

Fig. 1

Changes in lipid concentrations of the 41 subjects at the five time points. (A) daytime changes in triglyceride, (B) daytime changes in total cholesterol, (C) daytime changes in LDL-C, (D) daytime changes in HDL-C, (E) daytime changes in apolipoprotein A1, and (F) daytime changes in apolipoprotein B. Subjects 1 to 21 were males and subjects 22 to 41 were females; each of the box plots presents the maximum value, upper quartile, median, lower quartile, and minimum value.

Abbreviations: LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol.

3. Daytime BV in lipid concentrations

Of the data from 41 subjects, two outliers (one case of TG and one case of TC duplicate sample test results), nine individual variance outliers (two cases of LDL-C, three cases of HDL-C, two cases of apo-A1, one case of apo-B, and one case of TC), and three individual mean outliers (one case of TC, one case of LDL-C, and one case of apo-B) were excluded. The daytime BV of the 41 subjects and the comparison with previous studies are presented in Table 4. The daytime BV was the highest for TG and was relatively lower for the other lipid parameters. The II of TG, TC, LDL-C, HDL-C, apo-A1, and apo-B was 0.70, 0.30, 0.24, 0.23, 0.18, and 0.16, respectively. The RCV of TG, TC, LDL-C, HDL-C, apo-A1, and apo-B was 69.5%, 10.2%, 12.6%, 11.4%, 7.9%, and 12.2%, respectively.

Table 4

Within-subject and between-subject biological variation in lipids (%) across studies

Analyte	Diurnal biological variation								Long-term biological variation‡
	Current study*				Chen et al [26]		Sennels et al [27]	Rivera et al [28]	Fraser et al [14]		Minchinela et al [18]		Chen et al [26]		Pineda-Tenor et al [29]
	Mean±SD	CVA (95% CIs)	CVI (95% CIs)	CVG (95% CIs)	CVI	CVG	CVI	CVI	CVI	CVG	CVI	CVG	CVI	CVG	CVI	CVG
TG	1.38±0.69	2.1 (1.8–2.4)	25 (22.2–28.6)	35.9 (25.5–47.7)	32.2	54.1	37	39.1	15.3	27.8	19.9	32.7	27.6	59.3	20.3	34
TC	3.99±0.58	1.1 (1.0–1.2)	3.5 (3.0–4.0)	11.8 (9.3–15.8)	3.8	15.4	4.8	4.4	5.8	11	5.95	15.3	7.9	13.1	6.1	15
LDL-C	2.12±0.61	1.1 (1.0–1.2)	4.4 (3.8–5.0)	18.7 (14.8–25.0)	8.2	22.8	10.4	8.6	/	/	7.8	20.4	9.9	22.5	/	/
HDL-C	1.15±0.21	1.8 (1.6–2.0)	3.7 (3.0–4.1)	15.8 (12.4–21.1)	12.1	18.3	2	5.3	/	/	7.3	21.2	11.6	19.1	/	/
apo-A1	1.58±0.22	1.7 (1.5–1.9)	2.3 (1.6–2.4)	12.8 (10.1–17.1)	6.7	8.6	/	4.1	6.6	13.8	6.5	13.4	6.6	10.4	/	/
apo-B	0.65±0.11	3.7 (3.3–4.2)	2.4†	14.8 (11.7–19.8)	7.8	26.4	/	4.9	8.3	25.7	6.9	22.8	10.9	19.1	/	/

*The calculation model for biological variation was based on Braga and Panteghini [13]. Five blood samples were collected from a total of 41 subjects, each sample was tested twice, and a total of 410 samples were acquired. All samples were tested on the same day; †CVI confidence intervals could not be calculated because the CVA was greater than the CVI for apo-B; ‡Fasting blood sample.

Abbreviations: 95% CIs, 95% confidence intervals; TG, triglyceride (mmol/L); TC, total cholesterol (mmol/L); LDL-C, low-density lipoprotein cholesterol (mmol/L); HDL-C, high-density lipoprotein cholesterol (mmol/L); apo-A1, apolipoprotein A1 (g/L); apo-B, apolipoprotein B (g/L); CVA, analytical coefficient of variation; CVI, within-subject biological variation; CVG, between-subject biological variation.

The TG concentrations of male subjects were higher than those of female subjects, while the HDL-C concentrations of male subjects were lower than those of female subjects (P<0.05). The daytime BV of lipid concentrations for male and female subjects is shown in Table 5. The TC, LDL-C, apo-A1, apo-B concentrations were not statistically different between the male and female subjects (P>0.05).

Table 5

The within-subject and between-subject daytime biological variation of lipid concentrations in male and female subjects

Analyte	Sex	Mean ± SD	CVI% (95% CIs)	CVG% (95% CIs)
Triglyceride (mmol/L)	Male	1.32 ± 0.81	24.6 (20.8–29.9)	31.8 (20.4–46.0)
	Female	1.13 ± 0.56	25.4 (21.5–30.9)	35.1 (23.1–50.8)
High-density lipoprotein cholesterol (mmol/L)	Male	1.03 ± 0.17	3.8 (3.0–4.6)	13.8 (10.3–19.9)
	Female	1.27 ± 0.28	3.5 (2.8–4.2)	13.0 (9.7–18.8)

Abbreviations: CVI, within-subject biological variation; CVG, between-subject biological variation; 95% CIs, 95% confidence intervals.

DISCUSSION

To date, many studies have derived BV data for several analytes [18]. However, the high heterogeneity of these study protocols necessarily affects the BV estimates obtained. To ensure the validity of the present results, we referred to recent guidelines [1319] for assessing BV when selecting subjects, collecting and testing samples, and performing statistical analyses.

Of the six lipid parameters, TG concentration showed the greatest change between the five time points, although these changes were not all statistically significant. The change in postprandial TG concentration was highest at 12:00, while other lipid parameters were slightly decreased in most subjects, consistent with previous reports [22]. The mean change in the maximum fasting and postprandial TG concentration was 0.3–0.5 mmol/L following normal food intake [222324]. However, studies that included oral fat tolerance tests in their protocol reported mean changes of 1.06–1.6 mmol/L [825]. The changes in postprandial TG concentration in our study were relatively smaller than those in previous studies. This might be related to the following factors: First, the Han Chinese diet consists mainly of cereals, vegetables, fruits, and dairy products [3031]; our subjects' diets were relatively low in fats. Second, the average BMI of our participants was lower than that in previous studies [2224]; BMI also has a strong positive correlation with TG concentration [3233]. Third, our study had a prospective design, while most previous studies used cross-sectional data.

BV can be defined according to time interval (e.g., daily, monthly, seasonal, or annual BV). In our study, the daytime BV of TC, HDL-C, LDL-C, apo-A1, and apo-B was relatively low, consistent with previous reports [3262728]. However, the daytime BV of TG was relatively high and was greater than the long-term BV in the fasting state [141829]. The main cause of the high daytime BV in TG pertained to dietary composition; dietary fat, which can be directly absorbed, has a considerable impact on TG concentration. Of note, the TG daytime BV in our study was lower than that reported by Chen et al [26] and the diurnal BV in a Danish study [27] and a Spanish study [28]. These differences could be caused by differences in 1) blood sampling time points across studies, which would directly affect the lipid test results; 2) subjects' dietary habits and ethnicities; and 3) study design and statistical methods adopted.

Postprandial TG was generally higher than fasting TG; however, approximately one-third of the subjects exhibited decreased TG at the postprandial time points. Marcovina et al [15] reported similar findings and suggested that the differences in postprandial lipid changes were related to individual enzyme and hormone levels. Another reason for the variation in our findings could be that our subjects were not provided with a standardized diet and instead consumed their usual diet. For example, one subject ate chicken feet, which resulted in an abnormally high TG concentration (up to 5.59 mmol/L). However, we believe that having subjects follow their usual diets would better reflect real settings, while a standardized diet would likely cause discomfort and other physiological changes.

We also found significant differences in mean TG and HDL-C concentrations between males and females. Estrogen can decrease TG concentration while increasing HDL-C concentration [3435]. TG and HDL-C CVI and CVG were quite similar between males and females and also similar to the values of the total sample, a finding consistent with previous reports [1529].

All lipid parameter IIs in our study were <0.6 except for TG (0.7). These analytes showed high distinctiveness, rendering the reference intervals barely useful for result interpretation. In these cases, the RCV provides objective tools for assessing the significance of differences in the serial results of an individual. For example, two non-fasting TG results exhibiting a difference greater than 69.5% can be considered clinically significant. Most Han Chinese individuals consume a relatively low-volume and simple breakfast with low fat content, compared with lunch and supper. As shown in Table 1, TG variation was low in the morning (approximately 15% at 9:00); however, it increased in the afternoon (>20% at 12:00, 15:00, and 18:30). In cases of suspected dyslipidemia, obtaining a non-fasting sample in the afternoon would facilitate the detection of abnormal results. In addition, when considering the variation in lipid results, clinicians should also consider the fasting duration of the patient.

Our study had a number of limitations. First, the subjects were predominantly young individuals; thus, our results may have been different if middle-aged or elderly individuals were included. Second, the subjects were ostensibly healthy volunteers and only a few had abnormal lipid concentrations. Third, no standardized meals were provided; hence, differences in dietary composition may have influenced the results. Moreover, the nighttime was not covered in our study design, and hence, nocturnal BV could not be detected.

In conclusion, we found that the changes in the six lipid parameters at the five time points were relatively small, thus confirming that fasting is not required prior to routine lipid tests. Specifically, the daytime changes in TC, HDL-C, LDL-C, apo-A1, and apo-B were not significantly different. However, the change in TG was the greatest; thus, in cases of abnormal postprandial TG concentrations, dietary factors and fasting duration should be considered when interpreting the results.