| Literature DB >> 29797256 |
Sridurga Mithraprabhu1,2, Andrew Spencer3,4,5.
Abstract
Circulating tumor DNA (ctDNA) analysis is currently gaining momentum as an innovative methodology for characterizing the tumor genome and monitoring therapeutic efficacy in the multifocal, genetically and spatially heterogeneous plasma cell malignancy, multiple myeloma (MM). Circulating cell-free DNA (cfDNA), which consists of a combination of DNA derived from both tumor and normal cells, is present in extracellular bodily fluids. The presence of ctDNA within this admixture has been demonstrated recently in MM. In this chapter, we describe the routinely utilized methodology for the extraction and longitudinal analysis of specific mutations present in ctDNA derived from peripheral blood plasma of MM patients.Entities:
Keywords: Cell-free DNA; Circulating cell-free nucleic acids; Circulating tumor DNA; Droplet digital PCR; Liquid biopsy; Multiple myeloma; Peripheral blood plasma
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Year: 2018 PMID： 29797256 DOI： 10.1007/978-1-4939-7865-6_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745