Emilie Fréalle1,2, Philippe Gosset1, Sophie Leroy2, Claire Delattre3, Agnès Wacrenier3, Christoph Zenzmaier4, Christophe Zawadzki5, El Moukhtar Aliouat1,6, Susanne Perkhofer4. 1. Univ Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Center for Infection & Immunity of Lille, F-59000 Lille, France. 2. CHU Lille, Laboratoire de Parasitologie-Mycologie, F-59000 Lille, France. 3. CHU Lille, Laboratoire d'Anatomopathologie, F-59000 Lille, France. 4. Health University of Applied Sciences, Tyrol, Innsbruck, Austria. 5. CHU Lille, Laboratoire d'Hématologie & Univ. Lille, Inserm, Institut Pasteur, U1011, F-59000 Lille, France. 6. Laboratoire de Parasitologie, Faculté de Pharmacie de Lille - Univ Lille, France.
Abstract
AIM: To explore Aspergillus interactions with platelets in the blood, especially during clot formation. MATERIALS & METHODS: Aspergillus fumigatus resting or swollen conidia, germlings or hyphae were inoculated into blood sampled into tubes with or without anticoagulant. Interactions were explored using microscopy, and chemokine levels were determined. RESULTS: Anatomopathological examination of the clot revealed conidia and germlings colocalization with platelet aggregates, and neutrophil recruitment around aggregates. Transmission electron microscopy showed conidia and hyphae surrounded by neutrophils. Increased CCL5 and CXCL4 when conidia or germlings but not hyphae were added suggested they could be involved in neutrophil recruitment around aggregates. CONCLUSION: These data suggest platelets could trigger coagulopathy and activate neutrophils during aspergillosis. They open up new perspectives for aspergillosis management.
AIM: To explore Aspergillus interactions with platelets in the blood, especially during clot formation. MATERIALS & METHODS:Aspergillus fumigatus resting or swollen conidia, germlings or hyphae were inoculated into blood sampled into tubes with or without anticoagulant. Interactions were explored using microscopy, and chemokine levels were determined. RESULTS: Anatomopathological examination of the clot revealed conidia and germlings colocalization with platelet aggregates, and neutrophil recruitment around aggregates. Transmission electron microscopy showed conidia and hyphae surrounded by neutrophils. Increased CCL5 and CXCL4 when conidia or germlings but not hyphae were added suggested they could be involved in neutrophil recruitment around aggregates. CONCLUSION: These data suggest platelets could trigger coagulopathy and activate neutrophils during aspergillosis. They open up new perspectives for aspergillosis management.
Authors: Mariolina Bruno; Intan M W Dewi; Vicky Matzaraki; Rob Ter Horst; Marina Pekmezovic; Berenice Rösler; Laszlo Groh; Rutger J Röring; Vinod Kumar; Yang Li; Agostinho Carvalho; Mihai G Netea; Jean-Paul Latgé; Mark S Gresnigt; Frank L van de Veerdonk Journal: Comput Struct Biotechnol J Date: 2020-12-26 Impact factor: 7.271
Authors: Antonia Last; Michelle Maurer; Alexander S Mosig; Mark S Gresnigt; Bernhard Hube Journal: FEMS Microbiol Rev Date: 2021-09-08 Impact factor: 16.408