Luiza Helena S Almeida1, Rafael R Moraes1, Renata D Morgental1, Sérgio S Cava2, Wellington Luiz O Rosa1, Patrícia Rodrigues2, Anderson S Ribeiro3, Marcus Só4, Fernanda G Pappen5. 1. Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Brazil. 2. School of Materials Engineering, Federal University of Pelotas, Brazil. 3. Graduate Program in Chemistry, School of Chemistry, Laboratório de Metrologia Química (LabMeQui), Federal University of Pelotas, Brazil. 4. Graduate Program in Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Brazil. 5. Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Brazil. Electronic address: fernanda.pappen@ufpel.edu.br.
Abstract
OBJECTIVE: To synthetize calcium aluminate (C3A) and silver-containing C3A particles (C3A+Ag) testing their effects on the properties of a MTA-based endodontic sealer in comparison to an epoxy resin- and a calcium silicate-based sealer. METHODS: Pure C3A and C3A+Ag particles were synthesized by a chemical method and characterized using XRD to identify crystalline phases. SEM/EDS analysis investigated morphology, particle size, and elemental composition of particles. Setting time, flow, radiopacity, water sorption and solubility of commercial and modified sealers were evaluated according to ISO 6876/2012. The pH and ions release were measured using a pHmeter and a microwave induced plasma optical emission spectrometer. The inhibition of biofilm growth was evaluated by confocal laser scanning microscopy (CLSM). Data were rank transformed and analyzed by ANOVA and Tukey test (P<0.05). RESULTS: The C3A particles showed an irregular grain agglomerated structure with voids and pores. In C3A+Ag particles, Ag modified the material morphology, confirming the deposition of Ag. The physicochemical properties of the modified MTA-based sealer were similar to the commercial material, except for the significant increase in Ca+2 release. However, there was no Ag release. Setting time, flow, radiopacity, water sorption and solubility were adequate for all materials. All the materials showed alkaline pH. Antibiofilm effect was improved in the presence of C3A particles, while the biofilm inhibition was lower in the presence of Ag. SIGNIFICANCE: The modified sealer presented improved antibiofilm properties and calcium release, without dramatic effects on the other characteristics. It is expected a positive effect in its antimicrobial behavior.
OBJECTIVE: To synthetize calcium aluminate (C3A) and silver-containing C3A particles (C3A+Ag) testing their effects on the properties of a MTA-based endodontic sealer in comparison to an epoxy resin- and a calcium silicate-based sealer. METHODS: Pure C3A and C3A+Ag particles were synthesized by a chemical method and characterized using XRD to identify crystalline phases. SEM/EDS analysis investigated morphology, particle size, and elemental composition of particles. Setting time, flow, radiopacity, water sorption and solubility of commercial and modified sealers were evaluated according to ISO 6876/2012. The pH and ions release were measured using a pHmeter and a microwave induced plasma optical emission spectrometer. The inhibition of biofilm growth was evaluated by confocal laser scanning microscopy (CLSM). Data were rank transformed and analyzed by ANOVA and Tukey test (P<0.05). RESULTS: The C3A particles showed an irregular grain agglomerated structure with voids and pores. In C3A+Ag particles, Ag modified the material morphology, confirming the deposition of Ag. The physicochemical properties of the modified MTA-based sealer were similar to the commercial material, except for the significant increase in Ca+2 release. However, there was no Ag release. Setting time, flow, radiopacity, water sorption and solubility were adequate for all materials. All the materials showed alkaline pH. Antibiofilm effect was improved in the presence of C3A particles, while the biofilm inhibition was lower in the presence of Ag. SIGNIFICANCE: The modified sealer presented improved antibiofilm properties and calcium release, without dramatic effects on the other characteristics. It is expected a positive effect in its antimicrobial behavior.
Authors: Roula El Hachem; Nada El Osta; Hala Sacre; Pascale Salameh; Elie Wassef; Guy Le Brun; Fabrice Pellen; Bernard Le Jeune; Maha H Daou; Issam Khalil; Marie Abboud Journal: Eur Endod J Date: 2022-06
Authors: C Pushpalatha; Vismaya Dhareshwar; S V Sowmya; Dominic Augustine; Thilla Sekar Vinothkumar; Apathsakayan Renugalakshmi; Amal Shaiban; Ateet Kakti; Shilpa H Bhandi; Alok Dubey; Amulya V Rai; Shankargouda Patil Journal: Front Bioeng Biotechnol Date: 2022-08-09