Alison M McDermott1,2, Hasna Baidouri1, Ashley M Woodward3, Wendy R Kam3, Yang Liu3, Xiaomin Chen3, Jillian F Ziemanski4, Kerry Vistisen5, Linda D Hazlett5, Kelly K Nichols4, Pablo Argüeso3, David A Sullivan3. 1. a The Ocular Surface Institute , University of Houston College of Optometry , Houston , TX , USA. 2. e Department of Applied Sciences , Northumbria University , Newcastle upon Tyne , UK. 3. b Schepens Eye Research Institute of Massachusetts Eye and Ear and Department of Ophthalmology , Harvard Medical School , Boston , MA , USA. 4. c Ocular Surface Research Institute of the Clinical Eye Research Facility, School of Optometry , University of Alabama at Birmingham , Birmingham , AL , USA. 5. d Department of Anatomy & Cell Biology , Wayne State University School of Medicine , Detroit , MI , USA.
Abstract
PURPOSE: The purpose of this study is to establish the short tandem repeat (STR) profiles of several human cell lines commonly used in ocular surface research. MATERIALS AND METHODS: Independently DNA was extracted from multiple passages of three human corneal epithelial cell lines, two human conjunctival epithelial cell lines and one meibomian gland cell line, from different laboratories actively involved in ocular surface research. The samples were then subjected to STR analysis on a fee-for-service basis in an academic setting and the data compared against that in available databases. RESULTS: The STR profiles for the human corneal epithelial cells were different among the three cell lines studied and for each line the profiles were identical across the samples provided by three laboratories. Profiles for the human conjunctival epithelial cells were different among the two cell lines studied. Profiles for the meibomian gland cell line were identical across the samples provided by three laboratories. No samples were contaminated by elements of other cell lines such as HeLa. CONCLUSIONS: This comprehensive study provides verification of STR profiles for commonly used human ocular surface cell lines that can now be used as a reference by others in the field to authenticate the cell lines in use in their own laboratories.
PURPOSE: The purpose of this study is to establish the short tandem repeat (STR) profiles of several human cell lines commonly used in ocular surface research. MATERIALS AND METHODS: Independently DNA was extracted from multiple passages of three humancorneal epithelial cell lines, two human conjunctival epithelial cell lines and one meibomian gland cell line, from different laboratories actively involved in ocular surface research. The samples were then subjected to STR analysis on a fee-for-service basis in an academic setting and the data compared against that in available databases. RESULTS: The STR profiles for the humancorneal epithelial cells were different among the three cell lines studied and for each line the profiles were identical across the samples provided by three laboratories. Profiles for the human conjunctival epithelial cells were different among the two cell lines studied. Profiles for the meibomian gland cell line were identical across the samples provided by three laboratories. No samples were contaminated by elements of other cell lines such as HeLa. CONCLUSIONS: This comprehensive study provides verification of STR profiles for commonly used human ocular surface cell lines that can now be used as a reference by others in the field to authenticate the cell lines in use in their own laboratories.
Entities:
Keywords:
Conjunctiva; cornea; epithelial; meibomian; short tandem repeat
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