Literature DB >> 29786885

Automated Buildup of Biomimetic Films in Cell Culture Microplates for High-Throughput Screening of Cellular Behaviors.

Paul Machillot1,2, Catarina Quintal1,2, Fabien Dalonneau1,2, Loic Hermant1,2, Pauline Monnot1,2, Kelsey Matthews1,2, Vincent Fitzpatrick1,2, Jie Liu1,2, Isabelle Pignot-Paintrand1,2, Catherine Picart1,2.   

Abstract

An automatic method is established for layer-by-layer (LbL) assembly of biomimetic coatings in cell culture microplates using a commercial liquid-handling robot. Highly homogeneous thin films are formed at the bottom of each microwell. The LbL film-coated microplates are compatible with common cellular assays, using microplate readers and automated microscopes. Cellular adhesion is screened on crosslinked and peptide-functionalized LbL films and stem cell differentiation in response to increasing doses of bone morphogenetic proteins (2, 4, 7, 9). This method paves the way for future applications of LbL films in cell-based assays for regenerative medicine and high-throughput drug screening.
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  automation; cell culture plate; high content screening; layer-by-layer assembly; reproducibility; robot

Mesh：

Year:  2018        PMID： 29786885      PMCID： PMC6701983          DOI： 10.1002/adma.201801097

Source DB:  PubMed          Journal:  Adv Mater        ISSN： 0935-9648            Impact factor:   30.849


  37 in total

1.  Molecular basis for the explanation of the exponential growth of polyelectrolyte multilayers.

Authors:  C Picart; J Mutterer; L Richert; Y Luo; G D Prestwich; P Schaaf; J-C Voegel; P Lavalle
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-17       Impact factor: 11.205

2.  Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells.

Authors:  Daniel G Anderson; Shulamit Levenberg; Robert Langer
Journal:  Nat Biotechnol       Date:  2004-06-13       Impact factor: 54.908

3.  Measurement of film thickness up to several hundreds of nanometers using optical waveguide lightmode spectroscopy.

Authors:  Catherine Picart; Csilla Gergely; Youri Arntz; Jean-Claude Voegel; Pierre Schaaf; Frédéric J G Cuisinier; Bernard Senger
Journal:  Biosens Bioelectron       Date:  2004-10-15       Impact factor: 10.618

4.  Polyelectrolyte multilayers with a tunable Young's modulus: influence of film stiffness on cell adhesion.

Authors:  Aurore Schneider; Grégory Francius; Rodolphe Obeid; Pascale Schwinté; Joseph Hemmerlé; Benoît Frisch; Pierre Schaaf; Jean-Claude Voegel; Bernard Senger; Catherine Picart
Journal:  Langmuir       Date:  2006-01-31       Impact factor: 3.882

5.  Matrix elasticity directs stem cell lineage specification.

Authors:  Adam J Engler; Shamik Sen; H Lee Sweeney; Dennis E Discher
Journal:  Cell       Date:  2006-08-25       Impact factor: 41.582

6.  Glycated polyelectrolyte multilayer films: differential adhesion of primary versus tumor cells.

Authors:  Aurore Schneider; Anne-Laure Bolcato-Bellemin; Gregory Francius; Justyna Jedrzejwska; Pierre Schaaf; Jean-Claude Voegel; Benoit Frisch; Catherine Picart
Journal:  Biomacromolecules       Date:  2006-10       Impact factor: 6.988

7.  Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells.

Authors:  Hue H Luu; Wen-Xin Song; Xiaoji Luo; David Manning; Jinyong Luo; Zhong-Liang Deng; Katie A Sharff; Anthony G Montag; Rex C Haydon; Tong-Chuan He
Journal:  J Orthop Res       Date:  2007-05       Impact factor: 3.494

8.  Automated process for improved uniformity and versatility of layer-by-layer deposition.

Authors:  K C Krogman; N S Zacharia; S Schroeder; P T Hammond
Journal:  Langmuir       Date:  2007-02-09       Impact factor: 3.882

9.  Combinatorial signaling microenvironments for studying stem cell fate.

Authors:  Christopher J Flaim; Dayu Teng; Shu Chien; Sangeeta N Bhatia
Journal:  Stem Cells Dev       Date:  2008-02       Impact factor: 3.272

10.  Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments.

Authors:  Adam J Engler; Maureen A Griffin; Shamik Sen; Carsten G Bönnemann; H Lee Sweeney; Dennis E Discher
Journal:  J Cell Biol       Date:  2004-09-13       Impact factor: 10.539
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  4 in total

1.  Integrin-based adhesion compartmentalizes ALK3 of the BMPRII to control cell adhesion and migration.

Authors:  Olivier Destaing; Catherine Picart; Corinne Albiges-Rizo; Amaris Guevara-Garcia; Laure Fourel; Ingrid Bourrin-Reynard; Adria Sales; Christiane Oddou; Mylène Pezet; Olivier Rossier; Paul Machillot; Line Chaar; Anne-Pascale Bouin; Gregory Giannone
Journal:  J Cell Biol       Date:  2022-10-07       Impact factor: 8.077

2.  Design of experiments to assess the effect of culture parameters on the osteogenic differentiation of human adipose stromal cells.

Authors:  Mirasbek Kuterbekov; Paul Machillot; Francis Baillet; Alain M Jonas; Karine Glinel; Catherine Picart
Journal:  Stem Cell Res Ther       Date:  2019-08-14       Impact factor: 6.832

Review 3.  Debaryomyces hansenii: an old acquaintance for a fresh start in the era of the green biotechnology.

Authors:  Clara Navarrete; Mònica Estrada; José L Martínez
Journal:  World J Microbiol Biotechnol       Date:  2022-04-28       Impact factor: 4.253

Review 4.  Automation and data-driven design of polymer therapeutics.

Authors:  Rahul Upadhya; Shashank Kosuri; Matthew Tamasi; Travis A Meyer; Supriya Atta; Michael A Webb; Adam J Gormley
Journal:  Adv Drug Deliv Rev       Date:  2020-11-24       Impact factor: 15.470
  4 in total

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