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Literature DB >> 29785422

Super-resolution force spectroscopy reveals ribosomal motion at sub-nucleotide steps.

Abstract

Probing biomolecular motion beyond a single nucleotide is technically challenging but fundamentally significant. We have developed super-resolution force spectroscopy (SURFS) with 0.5 pN force resolution and revealed that the ribosome moves by half a nucleotide upon the formation of the pre-translocation complex, which is beyond the resolution of other techniques.

Entities: Chemical Gene

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Year: 2018 PMID： 29785422 PMCID： PMC5989005 DOI： 10.1039/c8cc02658k

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

32 in total

1. Recognition of cognate transfer RNA by the 30S ribosomal subunit.

Authors: J M Ogle; D E Brodersen; W M Clemons ; M J Tarry; A P Carter; V Ramakrishnan
Journal: Science Date: 2001-05-04 Impact factor: 47.728

2. EF-G-independent reactivity of a pre-translocation-state ribosome complex with the aminoacyl tRNA substrate puromycin supports an intermediate (hybrid) state of tRNA binding.

Authors: Divya Sharma; Daniel R Southworth; Rachel Green
Journal: RNA Date: 2004-01 Impact factor: 4.942

Super-resolution force spectroscopy reveals ribosomal motion at sub-nucleotide steps.

1. Recognition of cognate transfer RNA by the 30S ribosomal subunit.

2. EF-G-independent reactivity of a pre-translocation-state ribosome complex with the aminoacyl tRNA substrate puromycin supports an intermediate (hybrid) state of tRNA binding.

3. Direct observation of base-pair stepping by RNA polymerase.

4. Force-induced remnant magnetization spectroscopy for specific magnetic imaging of molecules.

5. Acoustofluidics 23: acoustic manipulation combined with other force fields.

6. A steric block in translation caused by the antibiotic spectinomycin.

7. Direct measurement of the mechanical work during translocation by the ribosome.

8. Multiperspective smFRET reveals rate-determining late intermediates of ribosomal translocation.

9. Visualization of the hybrid state of tRNA binding promoted by spontaneous ratcheting of the ribosome.

10. High-Efficiency "-1" and "-2" Ribosomal Frameshiftings Revealed by Force Spectroscopy.

1. Probing drug-DNA interactions using super-resolution force spectroscopy.

2. Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution.

3. High-Resolution DNA Dual-Rulers Reveal a New Intermediate State in Ribosomal Frameshifting.

4. Twice exploration of tRNA +1 frameshifting in an elongation cycle of protein synthesis.

Review 5. Advances in the Biological Application of Force-Induced Remnant Magnetization Spectroscopy.