Literature DB >> 2978377

Artificial dimers of native actin: preparation and properties in biological functions.

H G Bäumert1, A Kenmoku, G Middelhoff, F Ortanderl, A Thrun, H Faulstich, W Schiebler, H Fasold.   

Abstract

With the aid of tartryl-bis-epsilon-aminocaprylazide artificial dimers were produced from F actin from rabbit striated muscle. These derivatives will not polymerize by themselves but are able to copolymerize fully with native G actin. By modification of a single side chain per dimer, this copolymerization was completely inhibited. The dimers are able to activate subfragment 1 ATPase of myosin and bind to DNase I with inactivation of the enzyme in the same manner as native G actin. Within the dimer, one ADP is immobilized and will exchange against ATP extremely slowly. The dimers do not bind to the mushroom toxin phalloidin.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2978377     DOI： 10.1007/bf01024875

Source DB:  PubMed          Journal:  J Protein Chem        ISSN： 0277-8033


  15 in total

1.  Interaction of actin with phalloidin: polymerization and stabilization of F-actin.

Authors:  P Dancker; I Löw; W Hasselbach; T Wieland
Journal:  Biochim Biophys Acta       Date:  1975-08-19

2.  Spectroscopic evidence for the interaction of phalloidin with actin.

Authors:  T Wieland; J X de Vries; A Schäfer; H Faulstich
Journal:  FEBS Lett       Date:  1975-06-01       Impact factor: 4.124

3.  Head to tail polymerization of actin.

Authors:  A Wegner
Journal:  J Mol Biol       Date:  1976-11       Impact factor: 5.469

4.  A specific 1:1 G-actin:DNAase i complex formed by the action of DNAase I on F-actin.

Authors:  H G Mannherz; J B Leigh; R Leberman; H Pfrang
Journal:  FEBS Lett       Date:  1975-12-01       Impact factor: 4.124

5.  Studies on the structure of myosin.

Authors:  S LOWEY; C COHEN
Journal:  J Mol Biol       Date:  1962-04       Impact factor: 5.469

6.  The use of a new series of cleavable protein-crosslinkers on the Escherichia coli ribosome.

Authors:  L C Lutter; F Ortanderl; H Fasold
Journal:  FEBS Lett       Date:  1974-11-15       Impact factor: 4.124

7.  An actin-binding protein from Acanthamoeba regulates actin filament polymerization and interactions.

Authors:  G Isenberg; U Aebi; T D Pollard
Journal:  Nature       Date:  1980-12-04       Impact factor: 49.962

8.  Isolation of native microtubules from porcine brain and characterization of SH groups essential for polymerization at the GTP binding sites.

Authors:  K Mann; M Giesel; H Fasold; W Haase
Journal:  FEBS Lett       Date:  1978-08-01       Impact factor: 4.124

9.  The selective blocking of the polymerization reaction of striated muscle actin leading to a derivative suitable for crystallization. Modification of Tyr-53 by 5-diazonium-(1H)tetrazole.

Authors:  N Bender; H Fasold; A Kenmoku; G Middelhoff; K E Volk
Journal:  Eur J Biochem       Date:  1976-04-15

10.  Actin is the naturally occurring inhibitor of deoxyribonuclease I.

Authors:  E Lazarides; U Lindberg
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.