| Literature DB >> 29781598 |
Alexander R Jafek, Sean Harbertson, Hayden Brady, Raheel Samuel, Bruce K Gale.
Abstract
A microfluidic PCR device was developed that enables DNA amplification at speeds as fast as 2 s/cycle, with concurrent detection and amplification. Two targets were amplified from human genomic DNA. By observing the fluorescence emitted by a DNA dye while the sample is amplified, it is possible to obtain both qPCR and spatial melting information about the amplified product. The speed and integration of the device make it conducive to while-you-wait diagnostic tests that do not require post-PCR analysis.Entities:
Mesh：
Substances：
Year: 2018 PMID： 29781598 DOI： 10.1021/acs.analchem.7b05176
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986