| Literature DB >> 29777507 |
Marcello Rattazzi1,2,3, Elisabetta Faggin4, Elisa Bertacco4, Roberta Buso4,5, Massimo Puato4, Mario Plebani4, Martina Zaninotto4, Davide Condotta4, Giacomo Zoppellaro4, Leopoldo Pagliani6, Giuseppe Tarantini7, Sabino Iliceto7, Elisa Covolo7, Giuseppe Faggian8, Francesco Onorati8, Mikhail Dodonov8, Alessandro Daniotti9, Paola Pantano9, Zoran Olivari9, Giovanni Benfari10, Paolo Pauletto6.
Abstract
We aimed to investigate whether the expression of the OPG/RANK/RANKL triad in peripheral blood mononuclear cells (PBMC) and circulating levels of markers of ectopic mineralization (OPG, FGF-23, PPi) are modified in patients with calcific aortic valve disease (CAVD). We found that patients affected by CAVD (n = 50) had significantly higher circulating levels of OPG as compared to control individuals (p = 0.003). No differences between the two groups were found in FGF-23 and PPi levels. RANKL expression was higher in the PBMC from CAVD patients (p = 0.018) and was directly correlated with the amount of valve calcification (p = 0.032). In vitro studies showed that treatment of valve interstitial cells (VIC) with RANKL plus phosphate was followed by increase in matrix mineralization (p = 0.001). In conclusion, RANKL expression is increased in PBMC of patients with CAVD, is directly correlated with the degree of valve calcification, and promotes pro-calcific differentiation of VIC.Entities:
Keywords: Aortic valve calcification; FGF-23; OPG; Pyrophosphate; RANKL
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Year: 2018 PMID： 29777507 DOI： 10.1007/s12265-018-9804-2
Source DB: PubMed Journal: J Cardiovasc Transl Res ISSN： 1937-5387 Impact factor: 4.132