David de Gonzalo-Calvo1, Alberto Dávalos2, Manuel Fernández-Sanjurjo3, Laura Amado-Rodríguez4, Susana Díaz-Coto5, Cristina Tomás-Zapico3, Ana Montero6, Ángela García-González6, Vicenta Llorente-Cortés7, Maria Eugenia Heras8, Araceli Boraita Pérez8, Ángel E Díaz-Martínez9, Natalia Úbeda6, Eduardo Iglesias-Gutiérrez10. 1. Institute of Biomedical Research of Barcelona (IIBB) - Spanish National Research Council (CSIC), Barcelona, Spain, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain; Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain; CIBERCV, Institute of Health Carlos III, Madrid, Spain. Electronic address: david.degonzalo@gmail.com. 2. Laboratory of Epigenetics of Lipid Metabolism, Madrid Institute for Advanced Studies (IMDEA)-Food, CEI UAM+CSIC, Madrid, Spain. 3. Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain. 4. Unidad de Cuidados Críticos Cardiológicos, Área del Corazón, Hospital Universitario Central de Asturias, HUCA, Spain; Fundación para la Investigación Biosanitaria del Principado de Asturias (FINBA), Grupo de Investigación Traslacional en el Paciente Crítico, Asturias, Spain; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Spain. 5. Department of Statistics, University of Oviedo, Oviedo, Spain. 6. Department of Pharmaceutical and Health Sciences, CEU San Pablo University, Madrid, Spain. 7. Institute of Biomedical Research of Barcelona (IIBB) - Spanish National Research Council (CSIC), Barcelona, Spain, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain; CIBERCV, Institute of Health Carlos III, Madrid, Spain. 8. Department of Cardiology, Sports Medicine Centre, Spanish Sports Health Protection Agency, Madrid, Spain. 9. Clinical Laboratory, Sports Medicine Centre, Spanish Sports Health Protection Agency, Madrid, Spain. 10. Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain; Department of Pharmaceutical and Health Sciences, CEU San Pablo University, Madrid, Spain; Universidad Autónoma de Chile, Santiago, Chile. Electronic address: iglesiaseduardo@uniovi.es.
Abstract
BACKGROUND: Circulating microRNAs (c-miRNAs) are mediators of intercellular communication with great potential as cardiac biomarkers. The analysis of c-miRNAs in response to physiological stress, such as exercise, would provide valuable information for clinical practice and a deeper understanding of the molecular response to physical activity. Here, we analysed for the first time the acute exercise response of c-miRNAs reported as biomarkers of cardiac disease in a well-characterized cohort of healthy active adults. METHODS: Blood samples were collected immediately before and after (0 h, 24 h, 72 h) a 10-km race, a half-marathon (HM) and a marathon (M). Serum RNA from 10-km and M samples was extracted and a panel of 74 miRNAs analysed using RT-qPCR. c-miRNA response was compared with a panel of nine cardiac biomarkers. Functional enrichment analysis was performed. Pre- and post-M echocardiographic analyses were carried out. RESULTS: Serum levels of all cardiac biomarkers were upregulated in a dose-dependent manner in response to exercise, even in the absence of symptoms or signs of cardiac injury. A deregulation in the profiles of 5 and 19 c-miRNAs was observed for 10-km and M, respectively. Each race induced a specific qualitative and quantitative alteration of c-miRNAs implicated in cardiac adaptions. Supporting their discriminative potential, a number of c-miRNAs previously associated with cardiac disease were undetectable or stable in response to exercise. Conversely, "pseudo-disease" signatures were also observed. CONCLUSIONS: c-miRNAs may be useful for the management of cardiac conditions in the context of acute aerobic exercise. TRANSLATIONAL ASPECTS OF THE WORK: Circulating microRNAs could offer incremental diagnostic value to established and emerging cardiac biomarkers, such as hs-cTnT or NT-proBNP, in those patients with cardiac dysfunction symptoms after an acute bout of endurance exercise. Furthermore, circulating miRNAs could also show "pseudo-disease" signatures in response to acute exercise. Clinical practitioners should be aware of the impact caused by exercise in the interpretation of miRNA data.
BACKGROUND: Circulating microRNAs (c-miRNAs) are mediators of intercellular communication with great potential as cardiac biomarkers. The analysis of c-miRNAs in response to physiological stress, such as exercise, would provide valuable information for clinical practice and a deeper understanding of the molecular response to physical activity. Here, we analysed for the first time the acute exercise response of c-miRNAs reported as biomarkers of cardiac disease in a well-characterized cohort of healthy active adults. METHODS: Blood samples were collected immediately before and after (0 h, 24 h, 72 h) a 10-km race, a half-marathon (HM) and a marathon (M). Serum RNA from 10-km and M samples was extracted and a panel of 74 miRNAs analysed using RT-qPCR. c-miRNA response was compared with a panel of nine cardiac biomarkers. Functional enrichment analysis was performed. Pre- and post-M echocardiographic analyses were carried out. RESULTS: Serum levels of all cardiac biomarkers were upregulated in a dose-dependent manner in response to exercise, even in the absence of symptoms or signs of cardiac injury. A deregulation in the profiles of 5 and 19 c-miRNAs was observed for 10-km and M, respectively. Each race induced a specific qualitative and quantitative alteration of c-miRNAs implicated in cardiac adaptions. Supporting their discriminative potential, a number of c-miRNAs previously associated with cardiac disease were undetectable or stable in response to exercise. Conversely, "pseudo-disease" signatures were also observed. CONCLUSIONS: c-miRNAs may be useful for the management of cardiac conditions in the context of acute aerobic exercise. TRANSLATIONAL ASPECTS OF THE WORK: Circulating microRNAs could offer incremental diagnostic value to established and emerging cardiac biomarkers, such as hs-cTnT or NT-proBNP, in those patients with cardiac dysfunction symptoms after an acute bout of endurance exercise. Furthermore, circulating miRNAs could also show "pseudo-disease" signatures in response to acute exercise. Clinical practitioners should be aware of the impact caused by exercise in the interpretation of miRNA data.
Authors: David de Gonzalo-Calvo; Germán Cediel; Christian Bär; Julio Núñez; Elena Revuelta-Lopez; Josep Gavara; César Ríos-Navarro; Vicenta Llorente-Cortes; Vicente Bodí; Thomas Thum; Antoni Bayes-Genis Journal: Sci Rep Date: 2018-10-11 Impact factor: 4.379
Authors: Maciej Tarnowski; Patrycja Tomasiak; Marta Tkacz; Katarzyna Zgutka; Katarzyna Piotrowska Journal: Genes (Basel) Date: 2022-08-17 Impact factor: 4.141
Authors: Alex Cleber Improta-Caria; Carolina Kymie Vasques Nonaka; Bruno Raphael Ribeiro Cavalcante; Ricardo Augusto Leoni De Sousa; Roque Aras Júnior; Bruno Solano de Freitas Souza Journal: Int J Mol Sci Date: 2020-07-14 Impact factor: 5.923