Literature DB >> 2977353

Coliphage lambda to terminator lowers the stability of messenger RNA in Escherichia coli hosts.

G Gross1, I Hollatz.   

Abstract

The effects of the transcription terminators to and tfd on the overall high-level expression of a human interferon-beta gene (IFN-beta) in Escherichia coli hosts were compared. Deletion mapping shows that mRNA lability is caused by sequences at or near the lambda terminator to stem-loop structure. Extensive RNA secondary structure in this region indicates a potential RNase III cleavage/binding site. In RNase III- E. coli hosts, IFN-beta synthesis is indeed considerably enhanced. The bacteriophage tfd terminator does not confer this mRNA labilization phenomenon. In all cases, RNA level and stability correlate with the level of IFN-beta synthesized in the cell. In the system described, ongoing translation stabilizes mRNA only moderately.

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Year:  1988        PMID： 2977353     DOI： 10.1016/0378-1119(88)90133-3

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  3 in total

1.  Multiple determinants of functional mRNA stability: sequence alterations at either end of the lacZ gene affect the rate of mRNA inactivation.

Authors:  C Petersen
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490

2.  Roles of RNase E, RNase II and PNPase in the degradation of the rpsO transcripts of Escherichia coli: stabilizing function of RNase II and evidence for efficient degradation in an ams pnp rnb mutant.

Authors:  E Hajnsdorf; O Steier; L Coscoy; L Teysset; P Régnier
Journal:  EMBO J       Date:  1994-07-15       Impact factor: 11.598

3.  The Bacteriophage Lambda CII Phenotypes for Complementation, Cellular Toxicity and Replication Inhibition Are Suppressed in cII-oop Constructs Expressing the Small RNA OOP.

Authors:  Karthic Rajamanickam; Sidney Hayes
Journal:  Viruses       Date:  2018-03-07       Impact factor: 5.048
  3 in total

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