Jianmin Liu1, Beibei Liu2, Yuanyuan Guo2, Zhijun Chen2, Wei Sun2, Wuyue Gao2, Hongliang Wu2, Yan Wang2. 1. Department of Urology, First Affiliated Hospital of Bengbu Medical College, Anhui Province, PR China. Electronic address: liujianminah@163.com. 2. Department of Urology, First Affiliated Hospital of Bengbu Medical College, Anhui Province, PR China.
Abstract
OBJECTIVES: To explore the biological function and mechanism of miR-199a-3p in clear cell renal cell carcinoma (CCRCC). METHODS: We investigated the expression of miR-199a-3p in CCRCC through quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Over expression of miR-199a-3p was performed in CCRCC cell lines, and cell growth curve, colony formation capacity, cell invasion, wound healing and cell apoptosis assay were used for investigating the roles of miR-199a-3p in CCRCC. RESULTS: The expression of miR-199a-3p in CCRCC tissues was significantly lower than that in para-carcinoma tissues. Functional assay showed that over expression of miR-199a-3p influenced cell growth, colony formation, cell invasion, cell migration and cell apoptosis in CCRCC cell lines. CONCLUSIONS: Our work suggested that miR-199a-3p was related to cell growth, colony formation, cell invasion, cell migration and cell apoptosis, which might act as a tumor suppressor in CCRCC.
OBJECTIVES: To explore the biological function and mechanism of miR-199a-3p in clear cell renal cell carcinoma (CCRCC). METHODS: We investigated the expression of miR-199a-3p in CCRCC through quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Over expression of miR-199a-3p was performed in CCRCC cell lines, and cell growth curve, colony formation capacity, cell invasion, wound healing and cell apoptosis assay were used for investigating the roles of miR-199a-3p in CCRCC. RESULTS: The expression of miR-199a-3p in CCRCC tissues was significantly lower than that in para-carcinoma tissues. Functional assay showed that over expression of miR-199a-3p influenced cell growth, colony formation, cell invasion, cell migration and cell apoptosis in CCRCC cell lines. CONCLUSIONS: Our work suggested that miR-199a-3p was related to cell growth, colony formation, cell invasion, cell migration and cell apoptosis, which might act as a tumor suppressor in CCRCC.
Authors: Ioannis Bantounas; Filipa M Lopes; Kirsty M Rooney; Adrian S Woolf; Susan J Kimber Journal: Stem Cell Reports Date: 2020-12-10 Impact factor: 7.765