Karoline Walscheid1,2, Lisa Neekamp2, Arnd Heiligenhaus1,3, Toni Weinhage2, Carsten Heinz1,3, Dirk Foell2. 1. a Department of Ophthalmology and Ophtha Lab, St. Franziskus-Hospital Muenster , Muenster , Germany. 2. b Department of Pediatric Rheumatology and Immunology, University Hospital Muenster , Muenster , Germany. 3. c Department of Ophthalmology, University of Duisburg-Essen , Duisburg , Germany.
Abstract
Purpose: To characterize peripheral blood T cells in juvenile idiopathic arthritis-associated uveitis (JIAU). Methods: Blood samples were taken from children with JIAU (n = 18), JIA without ocular involvement (n = 11), idiopathic anterior uveitis (IAU, n = 12), and healthy controls (n = 11). Cells were stained for T cell surface markers, and intracellular cytokine staining was performed after cell stimulation and analyzed by flow cytometry. Results: The Th1/Th2 ratio was increased in JIAU patients. Numbers of IL-13-expressing cells an level of IL-13 and IL-10 expression per cell were increased in all patient groups; whereas, percentages of IL-5-expressing T cells were decreased. Numbers of proinflammatory Th17 cells and T cells expressing CTLA-4 were increased in all patient groups; whereas, γ/δ T cell numbers were decreased. Results from JIA and IAU were similar. Conclusion: T cell subtypes and potential T cell function are altered in pediatric patients with uveitis and arthritis as compared to healthy children.
Purpose: To characterize peripheral blood T cells in juvenile idiopathic arthritis-associated uveitis (JIAU). Methods: Blood samples were taken from children with JIAU (n = 18), JIA without ocular involvement (n = 11), idiopathic anterior uveitis (IAU, n = 12), and healthy controls (n = 11). Cells were stained for T cell surface markers, and intracellular cytokine staining was performed after cell stimulation and analyzed by flow cytometry. Results: The Th1/Th2 ratio was increased in JIAU patients. Numbers of IL-13-expressing cells an level of IL-13 and IL-10 expression per cell were increased in all patient groups; whereas, percentages of IL-5-expressing T cells were decreased. Numbers of proinflammatory Th17 cells and T cells expressing CTLA-4 were increased in all patient groups; whereas, γ/δ T cell numbers were decreased. Results from JIA and IAU were similar. Conclusion: T cell subtypes and potential T cell function are altered in pediatric patients with uveitis and arthritis as compared to healthy children.
Authors: Roos A W Wennink; Aridaman Pandit; Anne-Mieke J W Haasnoot; Sanne Hiddingh; Viera Kalinina Ayuso; Nico M Wulffraat; Bas J Vastert; Timothy R D J Radstake; Joke H de Boer; Jonas J W Kuiper Journal: Front Immunol Date: 2020-09-17 Impact factor: 7.561
Authors: Roos A W Wennink; Viera Kalinina Ayuso; Weiyang Tao; Eveline M Delemarre; Joke H de Boer; Jonas J W Kuiper Journal: Transl Vis Sci Technol Date: 2022-02-01 Impact factor: 3.283