G Xu1, Y Zhang, N Li, J-B Zhang, R Xu. 1. Department of Oncology, PLA No. 148 Hospital, Zibo, China. xugang118a@163.com.
Abstract
OBJECTIVE: We aimed at investigating the effects of long non-coding RNA (lncRNA) CCHE1 on proliferation and apoptosis of gastric cancer cells. MATERIALS AND METHODS: qRT-PCR method was used to detect lncRNA CCHE1 in cancer cell lines. Cells with relative expression were selected to change the expression by transfecting with corresponding lentiviral vector, and nonsense transfection group and blank control group were also constructed; cell counting kit-8 (CCK-8) method was used to assess cell proliferation activity; colony formation assay was used to evaluate the rate of cell cloning; flow cytometry assay was used to determine the apoptosis rate and cell cycle. Western blot was utilized to detect the expressions of Bax and Bcl-2. RESULTS: Expression of lncRNA CCHE1 in the tumor cell lines was increased (p<0.05). Up-regulated lncRNA CCHE1 increased proliferative activity and colony formation capacity. However, down-regulated lncRNA CCHE1 decreased proliferative activity and colony formation capacity. Bcl-2 protein expression increased, but Bax protein expression decreased in lncRNA CCHE1 upregulation group (p<0.05); the effect was reversed in lncRNA CCHE1 down-regulation group (p<0.05). CONCLUSIONS: lncRNA CCHE1 may promote the proliferation of gastric cancer cells and inhibit cell apoptosis.
OBJECTIVE: We aimed at investigating the effects of long non-coding RNA (lncRNA) CCHE1 on proliferation and apoptosis of gastric cancer cells. MATERIALS AND METHODS: qRT-PCR method was used to detect lncRNA CCHE1 in cancer cell lines. Cells with relative expression were selected to change the expression by transfecting with corresponding lentiviral vector, and nonsense transfection group and blank control group were also constructed; cell counting kit-8 (CCK-8) method was used to assess cell proliferation activity; colony formation assay was used to evaluate the rate of cell cloning; flow cytometry assay was used to determine the apoptosis rate and cell cycle. Western blot was utilized to detect the expressions of Bax and Bcl-2. RESULTS: Expression of lncRNA CCHE1 in the tumor cell lines was increased (p<0.05). Up-regulated lncRNA CCHE1 increased proliferative activity and colony formation capacity. However, down-regulated lncRNA CCHE1 decreased proliferative activity and colony formation capacity. Bcl-2 protein expression increased, but Bax protein expression decreased in lncRNA CCHE1 upregulation group (p<0.05); the effect was reversed in lncRNA CCHE1 down-regulation group (p<0.05). CONCLUSIONS: lncRNA CCHE1 may promote the proliferation of gastric cancer cells and inhibit cell apoptosis.