Maria A Simonova1, Victor D Pivovarov1, Dmitry Y Ryazantsev1, Anna S Dolgova2, Valentina M Berzhets3, Sergei K Zavriev1, Elena V Svirshchevskaya1. 1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 16/10, Miklukho-Maklaya St., 117997, Moscow, Russian Federation. 2. Central Research Institute of Epidemiology, 3A, Novogireyevskaya St., 111123, Moscow, Russian Federation. 3. Mechnikov's Institute of Vaccines & Sera, Russian Academy of Medical Sciences, Maliy Kazenny Pereulok, 5A, 105064 Moscow, Russian Federation.
Abstract
AIM: Estimation of specific IgE is essential for the prevention of allergy progression. Quantitative immuno-PCR (qiPCR) can increase the sensitivity of IgE detection. We aimed to develop qiPCR and compare it to the conventional ELISA in identification of IgE to Alt a 1 and Fel d 1 allergens. RESULTS: Single stranded 60-mer DNA conjugated to streptavidin was used to detect antigen-IgE-biotin complex by qiPCR. In semi-logarithmic scale qiPCR data were linear in a full range of serum dilutions resulting in three- to ten-times higher sensitivity of qiPCR in comparison with ELISA in IgE estimation in low titer sera. CONCLUSION: Higher sensitivity of qiPCR in identification of low titer IgE is a result of a higher linearity of qiPCR data.
AIM: Estimation of specific IgE is essential for the prevention of allergy progression. Quantitative immuno-PCR (qiPCR) can increase the sensitivity of IgE detection. We aimed to develop qiPCR and compare it to the conventional ELISA in identification of IgE to Alt a 1 and Fel d 1 allergens. RESULTS: Single stranded 60-mer DNA conjugated to streptavidin was used to detect antigen-IgE-biotin complex by qiPCR. In semi-logarithmic scale qiPCR data were linear in a full range of serum dilutions resulting in three- to ten-times higher sensitivity of qiPCR in comparison with ELISA in IgE estimation in low titer sera. CONCLUSION: Higher sensitivity of qiPCR in identification of low titer IgE is a result of a higher linearity of qiPCR data.
Entities:
Keywords:
Alt a 1; ELISA; Fel d 1; IgE; allergy; quantitative immuno-PCR; recombinant allergens
Authors: Artem V Maerle; Maria A Simonova; Victor D Pivovarov; Daria V Voronina; Polina E Drobyazina; Dmitriy Yu Trofimov; Leonid P Alekseev; Sergei K Zavriev; Dmitriy Yu Ryazantsev Journal: PLoS One Date: 2019-01-04 Impact factor: 3.240