INTRODUCTION: Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil. METHODS: An active entomologic research was conducted to identify adult specimens through an external morphology dichotomous key. The analysis of natural infection by T. cruzi in the insects was performed by isolation in culture and polymerase chain reaction. The antibody testing for T. cruzi in dogs was performed by enzyme-linked immunosorbent assay and indirect immunofluorescence assay. RESULTS: A total of 68 triatomines were captured, predominantly the Triatoma brasiliensis brasiliensis (Neiva 1911) species. The vector mapping displayed areas with greater risk for parasite transmission. Of the examined triatomines (51 specimens), 41.2% (21/51) were positive on polymerase chain reaction, and all were negative on culture. In the serum testing, 11% (25/218) of dogs were seropositive, but no association was found between the serologic results and the presence and infection by T. cruzi in triatomines. CONCLUSIONS: This study demonstrated the movement of T. cruzi in the studied area, by the presence of vectors and naturally infected domestic reservoirs. The mapping of the studied rural area demonstrates the risk of disease transmission.
INTRODUCTION:Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil. METHODS: An active entomologic research was conducted to identify adult specimens through an external morphology dichotomous key. The analysis of natural infection by T. cruzi in the insects was performed by isolation in culture and polymerase chain reaction. The antibody testing for T. cruzi in dogs was performed by enzyme-linked immunosorbent assay and indirect immunofluorescence assay. RESULTS: A total of 68 triatomines were captured, predominantly the Triatoma brasiliensis brasiliensis (Neiva 1911) species. The vector mapping displayed areas with greater risk for parasite transmission. Of the examined triatomines (51 specimens), 41.2% (21/51) were positive on polymerase chain reaction, and all were negative on culture. In the serum testing, 11% (25/218) of dogs were seropositive, but no association was found between the serologic results and the presence and infection by T. cruzi in triatomines. CONCLUSIONS: This study demonstrated the movement of T. cruzi in the studied area, by the presence of vectors and naturally infected domestic reservoirs. The mapping of the studied rural area demonstrates the risk of disease transmission.
Authors: Vinícius Viana Abreu Montanaro; Thiago Falcão Hora; Creuza Maria da Silva; Carla Verônica de Viana Santos; Maria Inacia Ruas Lima; Eleonora Maria de Jesus Oliveira; Gabriel R de Freitas Journal: Neurol Sci Date: 2019-07-30 Impact factor: 3.307
Authors: G E Benítez-Villa; A López-Monteon; E Waleckx; E Dumonteil; A J Márquez-Fernández; M J Rovirosa-Hernández; F Orduña-García; D Guzmán-Gómez; A Ramos-Ligonio Journal: Acta Parasitol Date: 2022-05-10 Impact factor: 1.534
Authors: Marta Victoria Cardinal; Gustavo Fabián Enriquez; Natalia Paula Macchiaverna; Hernán Darío Argibay; María Del Pilar Fernández; Alejandra Alvedro; María Sol Gaspe; Ricardo Esteban Gürtler Journal: PLoS Negl Trop Dis Date: 2021-05-12
Authors: Sofía Ocaña-Mayorga; Juan José Bustillos; Anita G Villacís; C Miguel Pinto; Simone Frédérique Brenière; Mario J Grijalva Journal: Pathogens Date: 2021-01-07
Authors: Laura Ramírez; Luana Dias de Moura; Natalia Lopes Fontoura Mateus; Milene Hoehr de Moraes; Leopoldo Fabrício Marçal do Nascimento; Nailson de Jesus Melo; Lucas Bezerra Taketa; Tatiana Catecati; Samuel G Huete; Karla Penichet; Eliane Mattos Piranda; Alessandra Gutierrez de Oliveira; Mario Steindel; Manoel Barral-Netto; Maria do Socorro Pires E Cruz; Aldina Barral; Manuel Soto Journal: Parasite Epidemiol Control Date: 2019-11-21