Literature DB >> 2976728

Storage of spheroplasts at -70 degrees C for transfection with phi X174 RF DNA.

H V Malling1, M C Fater, J G Burkhart, S C Hardies, C A Hutchison, M H Edgell.   

Abstract

A method was developed for preparation of spheroplasts used for transfection with phi X174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at -70 degrees C.

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Year:  1987        PMID: 2976728     DOI: 10.1016/0735-0651(87)90014-8

Source DB:  PubMed          Journal:  Gene Anal Tech        ISSN: 0735-0651


  1 in total

1.  Site-specific mutagenesis in cells with normal DNA repair systems: transitions produced from DNA carrying a single O6-alkylguanine.

Authors:  R W Chambers
Journal:  Nucleic Acids Res       Date:  1991-05-11       Impact factor: 16.971

  1 in total

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