A Novel Neuroimaging Model to Predict Early Neurological Deterioration After Acute Ischemic Stroke.

OBJECTIVE: In acute ischemic stroke, early neurological deterioration (END) may occur in up to one-third of patients. However, there is still no satisfying or comprehensive predictive model for all the stroke subtypes. We propose a practical model to predict END using magnetic resonance imaging (MRI).
METHOD: Patients with anterior circulation infarct were recruited and they underwent an MRI within 24 hours of stroke onset. END was defined as an elevation of ≥2 points on the National Institute of Health Stroke Scale (NIHSS) within 72 hours of stroke onset. We examined the relationships of END to individual END models, including: A, infarct swelling; B, small subcortical infarct; C, mismatch; and D, recurrence.
RESULTS: There were 163 patients recruited and 43 (26.4%) of them had END. The END models A, B and C significantly predicted END respectively after adjusting for confounding factors (p=0.022, p=0.007 and p<0.001 respectively). In END model D, we examined all imaging predictors of Recurrence Risk Estimator (RRE) individually and only the "multiple acute infarcts" pattern was significantly associated with END (p=0.032). When applying END models A, B, C and D, they successfully predicted END (p<0.001; odds ratio: 17.5[95% confidence interval: 5.1- 60.8]), with 93.0% sensitivity, 60.0% specificity, 45.5% positive predictive value and 96.0% negative predictive value.
CONCLUSION: The results demonstrate that the proposed model could predict END in all stroke subtypes of anterior circulation infarction. It provides a practical model for clinical physicians to select high-risk patients for more aggressive treatment to prevent END. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

INTRODUCTION

In acute ischemic stroke, a number of patients suffer from worsening neurological deficits, commonly called Early Neurological Deterioration (END). END occurs in up to one-third of patients and it varies due to different definitions of END by inconsistent scales or timeframes [1]. No matter what definition for END is used, it often leads to greater mortality and functional disability.

Several mechanisms have been proposed to explain END in acute ischemic stroke, including failure of collaterals, clot progression, recurrent stroke, cerebral edema, seizures, hemorrhagic transformation or re-occlusion of a recanalised artery [2]. Among these, hemodynamic factors and perfusion abnormalities are likely to play a critical role in END [3], leading to the infarct growth in the same vascular territory. It is referred to as “progressive stroke”, which is usually irreversible irrespective of whether medical treatments are applied [4]. Therefore, it is important to develop a practical guide to predict which patients are at risk of END. The improvement of neuroimaging helps to sheds light on the prediction of END after ischemic stroke. Magnetic Resonance Imaging (MRI) is widely used in clinical practice, providing information about the intracranial and extracranial vessels by Magnetic Resonance (MR) angiography, infarct volume by Diffusion Weighted Imaging (DWI), and hemodynamic status by perfusion imaging.

Based on the neuroimaging, Alawneh et al. proposed an operational classification of END after anterior circulation stroke by different mechanisms: 1. large infarct core, with further vasogenic edema to surrounding tissue; 2. large asymptomatic oligemia, with further transformation into infarction; 3. new ischemic event at previously healthy tissue in different vascular territory [3]. However, there was no practical definition for their classification. Moreover, the END classification by Alawneh et al. could not predict END in acute lacunar stroke because of small infarct and hypoperfused areas. Current studies have proved that infarct locations, Branch Atheromatous Disease (BAD) and perfusion abnormalities play important roles in the development of END in lacunar infarction [5-7]. Therefore, we introduce new neuroimaging END models A-D based on recent MRI studies, including infarct swelling, small subcortical infarct, mismatch and recurrence (Fig. ). It represents the major underlying mechanisms of END, including cerebral edema, hemodynamic compromise and recurrent stroke.

The concept of END model A, “large infarct core”, is derived from studies of malignant Middle Cerebral Artery (MCA) infarction (MMI), defined as a large infarction resulting in neurological deterioration due to edematous swelling of brain tissue with mass effect to the surrounding normal tissue. The most common predictor in Computed Tomography (CT) is the hypodense area beyond 50% of MCA territory. The DWI provides a more accurate estimation for core infarct volume and several cut points with an infarct volume over 78ml were associated with END and malignant MCA infarction (Table ) [8-15]. The END model B, “small subcortical infarct”, is applied to evaluate END in small vessel disease, using specific infarction location and perfusion defect [5, 6]. The END model C, “mismatch”, uses the concept of perfusion-diffusion mismatch in MRI to predict END (Table ) [16-18]. The END model D, “recurrence”, indicates a new ischemic event using imaging predictors of Recurrence Risk Estimator (RRE) score [19, 20].

In this study, we aim to investigate whether the END model can predict END occurrence within 72 hours after acute ischemic stroke.

MATERIALS AND METHODS

Patients

The clinical and imaging data were from two prospective studies using MRI to predict END at Chang Gung Memorial Hospital. In the prospective studies, patients were eligible to participate if they were 18 years of age or older, had a clinical diagnosis of ischemic stroke without thrombolytic therapy, and could undergo a complete MRI protocol within 24 hours after the onset of stroke, which was defined as the last time the patient was known to be without any neurological deficits. Patients with intravenous recombinant tissue plasminogen activator (rt-PA) or intra-arterial thrombectomy were excluded since these interventions will restore the compromised perfusion and significantly prevent END. The exclusion criteria in the prospective studies were patients: (1) with contraindications for MRI studies or gadolinium injections; (2) in whom DWI demonstrated no acute ischemic stroke; (3) with an acute ischemic stroke in the territories of posterior circulation. The prospective studies were approved by the Institutional Review Board of Chang Gung Memorial Hospital, and all examinations were performed after obtaining written informed consent from the patients or appropriate family members.

In the prospective studies, the neurological deficits were prospectively evaluated using the National Institute of Health Stroke Scale (NIHSS) on admission by a stroke neurologist or study nurse who was blinded to the patient’s MRI. Data on age, sex, cigarette smoking status, and a medical history of hypertension, diabetes mellitus, hypercholesterolemia, atrial fibrillation, prior coronary artery disease and prior cerebrovascular disease were recorded. Systolic and diastolic blood pressure values, blood biochemistry and cell counts were determined on admission. Complete surveys for stroke etiologies including electrocardiogram, carotid and transcranial Doppler and/or echocardiography were performed. END was defined as an elevation of ≧2 points on NIHSS within 72 hours of stroke onset [21-23]. Clinical outcomes at 3 months were evaluated using the modified Rankin Scale (mRS) by a study nurse who was blinded to the patient’s brain imaging. A good outcome was defined as a mRS score of 2 or less, and a favorable outcome was defined as a mRS score of 0 or 1. Mortality at 3 months was also recorded.

MRI Protocol and Image Analysis

All data were collected using a 3 Tesla Siemens Verio MRI system (Siemens Medical System, Erlangen, Germany) with a 16-channel head coil. The first MRI protocol included axial DWI, axial T1- and T2 images, MR angiography, fluid-attenuated inversion recovery (FLAIR) imaging and dynamic susceptibility contrast perfusion imaging. The follow-up protocol, including DWI, axial T1- and T2 images, MR angiography and FLAIR imaging, was followed on the 7th day after stroke onset. The detail MRI protocol and post-processing analysis were same to our previous study [24]. The parameters of time to maximum of residual tissue (Tmax) and Cerebral Blood Flow (CBF) were used to evaluate the hemodynamics. The imaging data were evaluated by two experienced stroke neurologists (Y.C.H. and Y.T.P.) and one neuroradiologist (Y.H.T.).

Definition of END Model

The END model includes 4 types (Fig. ), with following definitions for positive END:

END A, infarct swelling: an acute infarction over 80ml in DWI.

END B, small subcortical infarct: a single subcortical infarction of less than 20mm in diameter in the territory of penetrating arteries, with an acute DWI lesion in the posterior limb of the internal capsule or posterior location of the centrum semiovale, combined with a visible perfusion defect in CBF.

END C, mismatch: (Tmax>6s volume)/(DWI infarct volume) ratio > 120% and a Tmax>6s volume>10ml.

END D, recurrence: imaging predictors in the RRE score, including multiple acute infarcts, simultaneous infarcts in different circulations, multiple infarcts of different ages and isolated cortical infarcts.

The selection flowchart for END models is shown in Fig. (.

Fig. (2)

Flow diagram of study participants for END models. A total of 163 patients were eligible to enter this study. First, all patients were evaluated for END model A and 9 patients were stratified. The others were selected for evaluation of END model B when a single subcortical infarction of less than 20mm in diameter in the territory of penetrating arteries in DWI was present. After the exclusion of patients with positive model END A (N=9) and small subcortical infarction (N=65), the remaining patients (N=89) were evaluated for END model C. After excluding patients who were positive for END model C (N=22), the others with negative END model C (N=67) were surveyed for END model D.

Statistical Analysis

All statistical analyses were performed using the Statistical Program for Social Sciences (SPSS) statistical software (version 18, Chicago, IL, USA). Continuous variables were expressed as means ± standard deviations or median and interquartile range. The differences between the two groups were analyzed with the Mann-Whitney U test or Student t-test after testing for normality. Categorical data were analyzed using Fisher’s exact or Pearson’s Chi-Square test, as appropriate. Cohen’s Kappa coefficient was used to examine the reliability between the image readers. A multivariate logistic regression model was constructed to adjust for baseline variables when a p-value <0.1 was found in the univariate analysis in each END model in the prediction of END. All p values were two-tailed and a p value < 0.05 was considered to be statistically significant.

Results

A total of 295 patients with suspected stroke within 24 hours of the onset of symptoms were evaluated during the study period. The selection flowchart is shown in Fig. (. A total of 163 eligible patients were recruited and the demographic data are shown in Table .

Among the 163 patients, there were 43 patients (26.4%) who had END within 72 hours after stroke onset. There were no differences in age, sex ratio, rate of stroke risk factors, blood pressure or sugar at arrival, and duration of onset to MRI between patients with or without END. Compared to patients without END, those with END had higher initial NIHSS (11.0 vs. 6.7, p=0.003), higher NIHSS on third day (14.0 vs. 5.0, p<0.001), larger initial infarction volume (45.0ml vs. 8.1ml, p=0.012), larger initial perfusion defect volume (Tmax >6s: 73.1ml vs. 6.6ml, p<0.001), larger final infarction volume (74.3ml vs. 11.4ml; p<0.001), less good and favorable outcomes (11.6% vs. 60.8%, p<0.001; 23.3% vs. 71.7%, p<0.001), and higher mortality rate (14.0% vs. 1.7%, p=0.001). The Cohen’s Kappa coefficient was 0.816 to predict END model B between 2 image readers.

Table shows the results of each END model in the prediction of END. The END models A, B and C significantly predicted END respectively after adjusting for confounding factors (p=0.022, odds ratio: 15.8; p=0.007, odds ratio: 23.6; p<0.001, odds ratio: 12.8). In END model C, 22 patients were predicted as positive END and 67 patients were predicted as negative END. The 67 patients were selected for END model D. We examined each imaging predictor of REE (Supplementary Table ) and only the “multiple acute infarcts” pattern was significantly associated with END (p=0.028). Therefore, “multiple acute infarcts” pattern was selected to define END model D and it significantly predicted END after adjusting for confounding factors (p=0.032; odds ratio: 7.2).

END model A had the highest specificity and positive predictive value (PPV), whereas END model B had the highest sensitivity and negative predictive value (NPV). When combined, END models A, B and C could predict END (p<0.001, odds ratio: 9.3) with 76.7% sensitivity, 76.7% specificity, 54.1% PPV and 90.2% NPV. When using END models A, B, C and D, END could be predicted (p<0.001; odds ratio: 17.5[95% confidence interval: 5.1–60.8]), with 93.0% sensitivity, 60.0% specificity, 45.5% PPV and 96.0% NPV.

Discussion

In this study, we propose a practical neuroimaging model to predict END in acute ischemic stroke, including infarction swelling, small subcortical infarct, mismatch and recurrence. The results demonstrate that the proposed model can predict END in all stroke subtypes of anterior circulation infarction. It provides a practical way for clinical physicians to select high-risk patients for more aggressive treatment to prevent END.

The END model A was well documented in MMI and early infarction volumes measured by DWI with cut points over 78ml all have high sensitivity and specificity to predict END [8-15]. END in the MMI trial was usually defined as the deterioration of consciousness with evidence of brain swelling. In addition, the early decompressive hemicraniectomy within 24 hours of stroke onset in patients with large MCA infarction was proved to reduce mortality and improve functional outcomes [25]. Furthermore, a large infarct core >100ml was associated with a worse outcome after reperfusion therapy due to brain swelling and hemorrhage transformation [26]. Therefore, for patients with END model A, early decompressive hemicraniectomy should be considered in selected patients but reperfused therapy should be prudently selected.

END model B, small subcortical infarct, is applied to evaluate END in small vessel disease. Several imaging predictors have been proposed to predict END including BAD [7], posterior location of centrum semiovale [5] and perfusion defect [6, 27, 28]. In this study, positive END model B was defined as an acute infarction in the posterior limb of the internal capsule or posterior location of the centrum semiovale, combined with a perfusion defect in CBF. It successfully predicts END with high sensitivity (91.7%) but the specificity (64.2%) and PPV (36.7%) were not satisfactory. Some patients with perfusion defects did not suffer from progressive motor deficits most likely because the perfusion defect and infarct growth did not involve the corticospinal tract. Nevertheless, given the high sensitivity in predicting END and less hemorrhagic complications to antithrombotic agents [29], the selected patients in END model B may merit careful consideration for aggressive treatment such as dual antiplatelet [30-32], statin [33] or short-term anticoagulant [34].

END model C used perfusion-diffusion mismatch to predict END. In acute ischemic stroke, the threshold of a 6s delay in Tmax is well adopted to salvage the penumbral tissue in reperfusion therapy [35-37] and was also proved effective to predict END (Table ) [16-18]. Several reasons may help explain these paradoxical findings. The most likely reason is that there is no absolute cut point between penumbra and oligemia because the ischemic tolerance of the neurons may be different [38]. Furthermore, the “benign oligemia” is not always benign, and it still runs the risk of transforming into an infarction [39]. Therefore, the threshold of Tmax˃6s may contain both penumbra and oligemia, resulting in END model C being highly associated with END. However, the sensitivity and PPV in this model were not satisfactory. Some patients with positive END model C did not suffer from END probably due to the further restoration of perfection defects. Additionally, some infarct growth may not be manifested in NIHSS, and this may be the reason for the relatively low PPV. END model C did not predict 9 END patients but 7 of them were classified as positive END model D. This may suggest that additional arterial emboli may account for this END. The impaired clearance of emboli may increase the risk of END despite the perfusion compromise which doesn’t meet the criteria of the 6s lag in Tmax [40]. Further studies are needed to determine the optimal criteria, including Tmax threshold, hypoperfused volume or hypoperfused/infarct ratio. END model C provides an opportunity to select high-risk patients for more aggressive medical treatment or further endovascular thrombectomy.

END type D refers a recurrence of stroke, especially from cardioembolism or artery-to-artery embolism. The RRE score had been confirmed useful in prediction of early recurrent stroke within 7 days or within 90 days after stroke onset [19, 20]. The imaging predictor of “multiple acute infarcts” significantly predicts END, and is more likely to result from artery-to-artery emboli and hemodynamic compromise in large artery atherosclerosis [41-43]. The other predictors of “simultaneous infarcts in different circulations” and “isolated cortical infarcts” are associated to cardioembolism [44, 45] but they did not significantly predict END, probably due to a low recurrence rate in the acute stage. Therefore, the criteria of “multiple acute infarcts” in END type D focus on the large artery atherosclerosis. In these patients with a high risk of END, aggressive medical treatment is necessary for intracranial stenosis, whereas aggressive medical treatment followed by early endarterectomy is warranted in instances of internal carotid stenosis with minor stroke [46, 47].

Our study has certain limitations. First, the models in our study may not be extended to patients with posterior circulation infarction or with reperfusion therapy. Second, the timeframe from stroke onset to MRI scan was up to 24 hours and the optimal time in the prevention of END may gradually pass. However, the perfusion-diffusion mismatch was proved up to 48 hours in nonhuman primates [48] and more than half of END developed after 24 hours following stroke onset. Therefore, substantial salvageable tissue may still exist in this time window to prevent END. Third, the patient number in our study is relatively small. Further studies are necessary to validate and modify our model. Furthermore, attempting to predict END with a single MRI scan may be oversimplifying the underlying pathophysiology since the hemodynamic change after a stroke is a dynamic process.

Conclusion

The new practical END models successfully predict END within 72 hours after stroke onset in patients with anterior circulation infarction. Although not satisfactory, the proposed models are the most comprehensive imaging predictors of END in ischemic stroke and provide clinical physicians opportunities to select patients for clinical trials in the prevention END.

Ethics Approval and Consent to Participate

The prospective studies were approved by the Institutional Review Board of Chang Gung Memorial Hospital.

Human and Animal Rights

No Animals were used for studies that are base of this research. All the humans used were in accordance with the ethical standards of the committee responsible for human experimentation (institutional and national), and with the Helsinki Declaration of 1975, as revised in 2013 (http://ethics.iit.edu/ecodes/node/3931).

Consent for Publication

All participants or their guardians gave their informed consent.

Table 1

MRI predictors for malignant middle cerebral artery infarction.

MRI Predictor	Number	Image Time	Outcome	Sensitivity/ Specificity/PPV/NPV	Odds Ratio (95% Confidence Interval) P value	Study
DWI volume >78 mlDWI volume >78 ml + NIHSS≧22 after 24 h	135	<24 hours	MMI 2†	59%/ 98%/ 89%/ 91%79%/ 96%/ 94%/ 85%	NA	Kruetzelmann et al. [11]
ADC<80% lesion volume >82 mlTTP lesion volume >162 ml	37	<6 hours	MMI 1*	87%/ 91%/ 82%/ 92%83%/ 75%/ 53%/ 86%	NA	Thomalla et al. [15]
DWI volume >82 ml	140	<6 hours	MMI 2†	52%/ 98%/ 88%/ 90%	59.8 (12.2–292.8)p < 0.001	Thomalla et al. [14]
DWI volume >87 mlDWI volume >87 ml + Hemi-ICD	116	<6 hours	MMI 2†	76%/ 93%/ 70%/ 95%67%/ 99%/ 93% /93%	p < 0.001p=0.002	Beck et al. [9]
DWI volume >89 ml	30	<6 hours	Elevation of NIHSS≥4 within 48hours	86%/ 96%/NA /NA	11.5 (2.31–57.1)p=0.0028	Arenillas et al. [8]
DWI volume >102 m	69	<48 hours	MMI 3‡	85%/ 91%/ NA /NA	p <0.01	Goto et al. [10]
DWI volume >145 ml	61	<14 hours	Herniation with deteriorated consciousness	86%/ 88%/NA /NA	NA	Park et al. [13]
DWI volume >145 ml	28	<14 hours	MMI 4§	100%/ 94%/ 91%/ 100%	p <0.0001	Oppenheim et al. [12]

Abbreviations: ADC: APPARENT DIFFUSION COEFFICIENT; DWI: Diffusion Weighted Imaging; Hemi-ICD = Hemi-Intercaudate Distance; MMI: Malignant Middle Cerebral Artery (MCA) Infarction; NA: Not Available; NIHSS: National Institutes of Health Stroke Scale; TTP: Time to Peak; Tmax: Time to Maximum of Residual Tissue.

* MMI 1: Decline level of consciousness of ≥1 on item 1a of the NIHSS, and >2/3 MCA territory with compression of lateral ventricles or midline shift.

† MMI 2: MMI 1 + NIHSS˃18

‡ MMI 3: Decline in consciousness by Glasgow Coma Scale score and large space-occupying infarction with midline shift.

§ MMI 4: Deterioration of neurological and consciousness status with clinical signs of uncal herniation and mass effect.

Table 2

Perfusion-diffusion mismatch in prediction of early neurological deterioration.

Predictor	Number	Image Time	END	Sensitivity/ Specificity/PPV/NPV	Odds Ratio (95% Confidence Interval); P Value	Study
(Tmax>4s – DWI) >10ml	137	<24 hours	Any clinical deterioration	77%/ 83%/ NA/ NA	NA	Asdaghi et al. [16]
(Tmax>6s/DWI) > 120%DWI < 100ml	49	<24 hours	NIHSS≧4 in 72 hours	80%/ 79.5%/ NA/ NA	17.0 (2.8~105.0); p<0.01	Hsu et al. [17]
(Tmax>6s/DWI) > 120%+ (Tmax>6s) > 10ml	464	<4.5 hours	NIHSS≧4 in 24 hours	60%/ 67% / 9.4%/ 96.7%	NA	Simonsen et al. [18]
(Tmax>6s) > 35ml				76%/ 70.4%/ 12.8%/ 98.1%	NA

Abbreviations: DWI: Diffusion Weighted Imaging; END: Early Neurological Deterioration; NA: Not Available; NIHSS: National Institutes of Health Stroke Scale; NPV: Negative Predictive Value; PPV: Positive Predictive Value; Tmax: Time to Maximum of Residual Tissue.

Table 3

Baseline characteristics, imaging findings and outcomes in patients with and without END.

	END N=43	No END N=120	p
Age (year)	72.6±14.6	70.6±10.9	p=0.061
Sex (Female)	18(41.9%)	55(45.8%)	p=0.653
BMI	24.3±3.9	24.7±3.9	p=0.491
Atrial fibrillation	13(30.2%)	29(24.2%)	p=0.435
Diabetes mellitus	21(48.8%)	53(44.2%)	p=0.598
Hypertension	36(83.7%)	95(79.2%)	p=0.519
Hyperlipidemia	32(74.4%)	85(70.8%)	p=0.654
Coronary artery disease	4(9.3%)	7(5.8%)	p=0.483
Old stroke	15(34.9%)	26(21.7%)	p=0.087
Smoking	8(18.6%)	31(25.8%)	p=0.340
Systolic blood pressure (mmHg)	176.7±39.0	169.5±34.0	p=0.397
Diastolic blood pressure (mmHg)	99.9±20.6	95.7±17.5	p=0.390
Sugar (mg/dL)	151.3±73.5	149.0±64.0	p=0.997
Onset-MRI duration (hour)	12.8±7.1	14.5±6.9	p=0.202
NIHSS baseline	11.0±8.6	6.7±5.8	p=0.003
NIHSS on 3rd day	14.0±9.6	5.0±5.4	p<0.001
Core infarct volume (ml)	45.0±87.6	8.1±18.2	p=0.012
Tmax >6s volume (ml)	73.1±118.3	6.6±21.3	p<0.001
Final infarct volume (ml)	74.3±120.6	11.4±24.8	p<0.001
Infarct growth (ml)	26.9±48.9	3.3±10.7	p<0.001
mRS at 3-months	3.7±1.7	1.5±1.8	p<0.001
Favorable outcome at 3-months	5(11.6%)	73(60.8%)	p<0.001
Good outcome at 3-months	10(23.3%)	86(71.7%)	p<0.001
Mortality at 3-months	6(14.0%)	2(1.7%)	p=0.001

All data was expressed as mean ± standard deviation or presented as counts and percentages.

Abbreviations:

BMI: Body Mass Index; END: Early Neurological Deterioration; MRI: Magnetic Resonance Imaging; NIHSS: National Institute of Health Stroke Scale; mRS: Modified Rankin Scale; Tmax: Time to Maximum of Residual Tissue.

Table 4

END model in prediction of early neurological deterioration.

END Model	Total Number	All END Number (%)	Predicted END Number	Successfully Predicted END Number	Parameters	p	Odds Ratio (95% Confidence Interval)	Sensitivity/ Specificity/PPV/NPV
A	163	43 (26.1%)	9	8	DWI > 80ml	0.0030.022*	25.6(3.1 – 211.8)15.8(1.5 – 166.0)*	18.6%/ 99.2%/ 88.9%/ 77.3%
B	65	12 (18.5%)	30	11	Perfusion defect in CBF maps + infarct location	0.0060.007†	19.7(2.4 – 164)23.6(2.4 – 233.5)†	91.7%/ 64.2%/ 36.7%/ 97.1%
C	89	23 (25.8%)	22	14	(Tmax>6s/DWI) > 120% + (Tmax>6s) > 10ml	<0.001<0.001‡	11.3(3.7 – 34.5)12.8(3.8 – 42.7) ‡	60.9%/ 87.9%/ 63.6%/ 86.6%
D	67	9 (14.3%)	28	7	Multiple acute infarcts	0.0320.032‡	6.2(1.2 – 32.4)7.2 (1.2 – 43.1)‡	77.8%/ 63.8%/ 25.0%/ 94.9%
A+B+C	163	43 (26.1%)	61	33	Models A+B+C	<0.001<0.001*	10.8(4.8 – 24.7)9.3(4.0 – 21.6) *	76.7%/ 76.7%/ 54.1%/ 90.2%
A+B+C+D	163	43 (26.1%)	89	40	Models A+B+C+D	<0.001<0.001*	20.0(5.9 – 68.3)17.5(5.1 – 60.8)*	93.0%/ 60.0%/ 45.5%/ 96.0%

Abbreviations:

DWI: Diffusion Weighted Imaging; END: Early Neurological Deterioration; NPV: Negative Predictive Value; PPV: Positive Predictive Value; Tmax: Time to Maximum of Residual Tissue.

A multivariate logistic regression model was constructed to adjust for baseline variables when a p-value <0.1 was found in the univariate analysis:

* Adjusted for old stroke and baseline NIHSS.

† Adjusted for old stroke and sex.

‡ Adjusted for smoking and sex.