Samaneh Gholami1,2, Nematolah Gheibi1, Reza Falak1, Koorosh Goodarzvand Chegini3. 1. Department of Biotechnology, Qazvin University of Medical Sciences, Qazvin, Iran. 2. Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran. 3. Department of Clinical Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, Iran.
Abstract
BACKGROUND: Betatrophin is a member of the angiopoietin-like (ANGPTL) family that has been implicated in both triglyceride and glucose metabolism. The physiological functions and molecular targets of this protein remain largely unknown; hence, a purified available protein would aid study of the exact role of betatrophin in lipid or glucose metabolism. METHODS: In this study, we cloned the full-length cDNA of betatrophin from a human liver cDNA library. Betatrophin was expressed in the pET-21b-E. coli Bl21 (DE3) system and purified by immobilized metal-affinity chromatography and ion-exchange chromatography. RESULTS: Circular dichroism spectroscopy revealed α-helix as the major regular secondary structure in recombinant betatrophin. CONCLUSION: The production method is based on commonly available resources; therefore, it can be readily implemented.
BACKGROUND: Betatrophin is a member of the angiopoietin-like (ANGPTL) family that has been implicated in both triglyceride and glucose metabolism. The physiological functions and molecular targets of this protein remain largely unknown; hence, a purified available protein would aid study of the exact role of betatrophin in lipid or glucose metabolism. METHODS: In this study, we cloned the full-length cDNA of betatrophin from a human liver cDNA library. Betatrophin was expressed in the pET-21b-E. coli Bl21 (DE3) system and purified by immobilized metal-affinity chromatography and ion-exchange chromatography. RESULTS: Circular dichroism spectroscopy revealed α-helix as the major regular secondary structure in recombinant betatrophin. CONCLUSION: The production method is based on commonly available resources; therefore, it can be readily implemented.
Entities:
Keywords:
CD spectroscopy; Human betatrophin; Recombinant protein
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