Francisco Javier Manzano-Moreno1, Victor Javier Costela-Ruiz2, Lucía Melguizo-Rodríguez3, Rebeca Illescas-Montes4, Olga García-Martínez3, Concepción Ruiz5, Javier Ramos-Torrecillas3. 1. Biomedical Group (BIO277), Department of Stomatology, School of Dentistry, University of Granada, Spain; Instituto Investigación Biosanitaria, ibs. Granada, Spain. 2. Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Spain. 3. Instituto Investigación Biosanitaria, ibs. Granada, Spain; Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Spain. 4. Instituto Investigación Biosanitaria, ibs. Granada, Spain; Biomedical Group (BIO277). Department of Nursing, Faculty of Health Sciences, Melilla, University of Granada, Spain. 5. Instituto Investigación Biosanitaria, ibs. Granada, Spain; Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Spain; Institute of Neuroscience, Parque Tecnológico Ciencias de la Salud, Armilla, Granada, University of Granada, Spain. Electronic address: crr@ugr.es.
Abstract
OBJECTIVE: To determine the effect of different nonsteroidal anti-inflammatory drugs (NSAIDs) on vascular endothelial growth factor (VEGF) gene expression in two osteoblast cell populations. DESIGN: Osteoblasts obtained by primary culture (HOp) and human osteosarcoma cell line MG63 (MG-63), which were treated with 10 μM doses of acetaminophen, indomethacin, ketoprofen, diclofenac, ibuprofen, ketorolac, naproxen or piroxicam. At 24 h of treatment, their gene expression of VEGF was evaluated by real-time polymerase chain reaction (RT-PCR) and compared with the expression in untreated cells (control group). RESULTS: The treatment with the different NSAIDs significantly reduced VEGF expression regardless of the cell line and NSAID studied. CONCLUSION: The results of this study suggest that these drugs may have undesirable effects on the osteoblast and its bone-forming capacity, given the effect of this growth factor on these cells. Further studies are warranted to determine their repercussions on bone tissue and to elucidate the cell signaling mechanism/s involved.
OBJECTIVE: To determine the effect of different nonsteroidal anti-inflammatory drugs (NSAIDs) on vascular endothelial growth factor (VEGF) gene expression in two osteoblast cell populations. DESIGN: Osteoblasts obtained by primary culture (HOp) and humanosteosarcoma cell line MG63 (MG-63), which were treated with 10 μM doses of acetaminophen, indomethacin, ketoprofen, diclofenac, ibuprofen, ketorolac, naproxen or piroxicam. At 24 h of treatment, their gene expression of VEGF was evaluated by real-time polymerase chain reaction (RT-PCR) and compared with the expression in untreated cells (control group). RESULTS: The treatment with the different NSAIDs significantly reduced VEGF expression regardless of the cell line and NSAID studied. CONCLUSION: The results of this study suggest that these drugs may have undesirable effects on the osteoblast and its bone-forming capacity, given the effect of this growth factor on these cells. Further studies are warranted to determine their repercussions on bone tissue and to elucidate the cell signaling mechanism/s involved.