Literature DB >> 2976326

A sensitive non-radioactive assay for pyrimidine nucleoside phosphorylase using reversed-phase high performance liquid chromatography.

E J Laurensse1, H M Pinedo, G J Peters.   

Abstract

A new sensitive assay for pyrimidine nucleoside phosphorylase using non-radioactive substrates is described. With the natural substrate uridine (UR) and the analog, 5'-deoxy-5-fluorouridine (5'dFUR) conditions have been optimized to measure the product formation with reversed-phase high-performance liquid chromatography. Using automated injection large series of samples may be analyzed. The assay for UR phosphorylase appeared to be comparable to existing methods with radiolabeled UR as substrate regarding sensitivity and linearity. The assay has been used to measure kinetic parameters for 5'dFUR and UR in two cell lines from intestinal origin.

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Year:  1988        PMID： 2976326     DOI： 10.1016/0009-8981(88)90270-7

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  4 in total

1.  Thymidine phosphorylase in cancer cells stimulates human endothelial cell migration and invasion by the secretion of angiogenic factors.

Authors:  I V Bijnsdorp; F Capriotti; F A E Kruyt; N Losekoot; M Fukushima; A W Griffioen; V L Thijssen; G J Peters
Journal:  Br J Cancer       Date:  2011-03-08       Impact factor: 7.640

2.  Cellular pharmacology of multi- and duplex drugs consisting of ethynylcytidine and 5-fluoro-2'-deoxyuridine.

Authors:  Irene V Bijnsdorp; Reto A Schwendener; Herbert Schott; Iduna Fichtner; Kees Smid; Adrie C Laan; Sarah Schott; Nienke Losekoot; Richard J Honeywell; Godefridus J Peters
Journal:  Invest New Drugs       Date:  2009-12-03       Impact factor: 3.850

3.  Enhanced therapeutic efficacy of 5'deoxy-5-fluorouridine in 5-fluorouracil resistant head and neck tumours in relation to 5-fluorouracil metabolising enzymes.

Authors:  G J Peters; B J Braakhuis; E A de Bruijn; E J Laurensse; M van Walsum; H M Pinedo
Journal:  Br J Cancer       Date:  1989-03       Impact factor: 7.640

4.  Role of platelet-derived endothelial cell growth factor/thymidine phosphorylase in fluoropyrimidine sensitivity.

Authors:  M de Bruin; T van Capel; K Van der Born; F A Kruyt; M Fukushima; K Hoekman; H M Pinedo; G J Peters
Journal:  Br J Cancer       Date:  2003-03-24       Impact factor: 7.640
  4 in total

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