| Literature DB >> 29762900 |
Mugui Wang1, Yanfei Mao1, Yuming Lu1, Zhidan Wang1, Xiaoping Tao1, Jian-Kang Zhu1,2.
Abstract
We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co-expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.Entities:
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Year: 2018 PMID: 29762900 DOI: 10.1111/jipb.12667
Source DB: PubMed Journal: J Integr Plant Biol ISSN: 1672-9072 Impact factor: 7.061